Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets.
Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets.
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利用神经活性中药方剂和生物靶点对斑马鱼进行行为筛查
DOI：
10.1038/srep05311
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发表时间：
2014-06-16
影响因子：
4.6
通讯作者：
Feng XZ
中科院分区：
文献类型：
--
作者：
Wang YN;Hou YY;Sun MZ;Zhang CY;Bai G;Zhao X;Feng XZ
抗抑郁药治疗抑郁症的作用机制在中医学中仍不清楚。在这项研究中，我们选择了7个经典的中医处方，并利用自动视频跟踪系统监测48小时的斑马鱼仔鱼在受精后4天（dpf）的休息/觉醒行为。结果表明，各方剂的疗效呈剂量依赖性。根据斑马鱼共有的行为表型进行K-均值聚类。结果显示，相同类别处方诱导的休息/清醒行为特征相似。并对中药方剂与已知化合物进行相关性分析。结果表明，中药方剂与一些知名化合物有较好的相关性。因此，我们预测它们可能具有相似的作用机制。本研究首次将联合收割机与斑马鱼的静息/觉醒行为研究相结合，为探索中药方剂的作用机制提供了一条有力的途径。
The mechanism of the therapeutic action of antidepressants remains uncertain in traditional Chinese medicine (TCM). In this study, we selected 7 classical TCM prescriptions and utilised an automatic video-tracking system to monitor the rest/wake behaviour of larval zebrafish at 4 days post-fertilisation (dpf) for 48 hours. We found that the curative effects of the prescriptions were dose-dependent. K-means clustering was performed according to the shared behavioural phenotypes of the zebrafish. The results revealed that the rest/wake behavioural profiles induced by the same class of prescriptions were similar. A correlation analysis was conducted between the TCM prescriptions and the known compounds. The results showed that the TCM prescriptions correlated well with some well-known compounds. Therefore, we predicted that they may share a similar mechanism of action. This paper describes the first study to combine TCM research with zebrafish rest/wake behaviour in vivo and presents a powerful approach for the discovery of the mechanism of action of TCM prescriptions.
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2.7
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通讯作者：
Peterson, Randall T.