Inorganic-Organic Interpenetrating Network Hydrogels as Tissue-Integrating Luminescent Implants: Physicochemical Characterization and Preclinical Evaluation.
Inorganic-Organic Interpenetrating Network Hydrogels as Tissue-Integrating Luminescent Implants: Physicochemical Characterization and Preclinical Evaluation.
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DOI：
10.1002/mabi.202100380
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发表时间：
2022-03
影响因子：
4.6
通讯作者：
McShane MJ
中科院分区：
文献类型：
--
作者：
Unruh RM;Bornhoeft LR;Nichols SP;Wisniewski NA;McShane MJ
能够准确、连续监测生物化学的传感器对于大规模实现个性化医疗至关重要。已经取得了很大的进步，以提高长期在体内生物传感器的组织相容性，使用生物材料的策略，如组织整合水凝胶。然而，组织中低水平的氧对植入设备提出了挑战，特别是当生物传感功能依赖于氧作为被测量物时-作为主要分析物或作为间接标记物来测量其他生物分子的水平。本工作提出了一种制备无机-有机互穿网络（IPN）水凝胶的方法，以优化氧气通过可注射生物传感器的传输。利用两种网络之间的协同作用，各种物理化学性质（例如溶胀，玻璃化转变温度和机械性能）被证明是独立可调的，同时保持相对于聚（2-羟乙基甲基丙烯酸酯）（pHEMA）控制的氧气渗透性增加250%。最后，当用Pd（II）苯并卟啉磷光体官能化时，这些凝胶作为猪模型中的皮下植入物在真实的时间内跟踪组织氧达76天，同时促进组织向内生长并使植入物周围的纤维化最小化。这些发现支持IPN网络用于个性化医疗和其他生物医学应用中可植入生物材料的微调设计。互穿网络（IPN）水凝胶是由无机和有机网络构成的光学生物传感器。两种聚合物网络之间的协同作用允许在优化氧传输的同时独立调节物理化学性质。具有倒置胶体晶体微结构的IPN传感器在猪模型中作为皮下植入物在真实的时间内跟踪组织氧76天，促进组织向内生长并最小化纤维化包裹。
Sensors capable of accurate, continuous monitoring of biochemistry are crucial to the realization of personalized medicine on a large scale. Great strides have been made to enhance tissue compatibility of long-term in vivo biosensors using biomaterials strategies such as tissue-integrating hydrogels. However, the low level of oxygen in tissue presents a challenge for implanted devices, especially when the biosensing function relies on oxygen as a measurand—either as a primary analyte or as an indirect marker to transduce levels of other biomolecules. This work presents a method of fabricating inorganic-organic interpenetrating network (IPN) hydrogels to optimize the oxygen transport through injectable biosensors. Capitalizing on the synergy between the two networks, various physicochemical properties (e.g. swelling, glass transition temperature, and mechanical properties) are shown to be independently adjustable while maintaining a 250% increase in oxygen permeability relative to poly(2-hydroxyethyl methacrylate) (pHEMA) controls. Finally, these gels, when functionalized with a Pd(II) benzoporphyrin phosphor, track tissue oxygen in real time for 76 days as subcutaneous implants in a porcine model while promoting tissue ingrowth and minimizing fibrosis around the implant. These findings support IPN networks for fine-tuned design of implantable biomaterials in personalized medicine and other biomedical applications. Interpenetrating network (IPN) hydrogels are fabricated with inorganic and organic networks as optical biosensors. Synergy between both polymer networks allows independent tuning of physicochemical properties while optimizing oxygen transport. IPN sensors with inverted colloidal crystal microarchitecture track tissue oxygen in real time for 76 days as subcutaneous implants in a porcine model, promoting tissue ingrowth and minimizing fibrotic encapsulation.
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DOI：
10.1097/icl.0000000000000033
发表时间：
2014-09-01
影响因子：
2.3
作者：
Guidi, Giuliano;Korogiannaki, Myrto;Sheardown, Heather
通讯作者：
Sheardown, Heather
影响因子：
5.4
作者：
Jacob S;Nair AB;Shah J;Sreeharsha N;Gupta S;Shinu P
通讯作者：
Shinu P
影响因子：
3.1
作者：
Kanick, Stephen C.;Schneider, Peter A.;Rebrin, Kerstin
通讯作者：
Rebrin, Kerstin
影响因子：
4.6
作者：
Chien, Jennifer S.;Mohammed, Mahmoud;Klitzman, Bruce
通讯作者：
Klitzman, Bruce
影响因子：
1.4
作者：
Lasowski, Frances;Sheardown, Heather
通讯作者：
Sheardown, Heather