The ecological consequences and evolution of retron-mediated suicide as a way to protect Escherichia coli from being killed by phage.
The ecological consequences and evolution of retron-mediated suicide as a way to protect Escherichia coli from being killed by phage.
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DOI：
10.1371/journal.pone.0285274
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
Levin, Bruce R.
中科院分区：
文献类型：
--
作者：
Berryhill, Brandon A.;Manuel, Joshua A.;Garcia, Rodrigo;Levin, Bruce R.
逆转录子在1984年被描述为编码逆转录酶和称为多拷贝单链DNA（msDNA）的独特单链DNA/RNA杂交体的DNA序列。直到2020年，才显示出逆转子的功能，当时有令人信服的证据表明逆转子激活了一个失败的感染途径，以应对噬菌体（噬菌体）感染。当被λ噬菌体的毒性突变体λVIR以及在较小程度上被其它λ噬菌体感染时，一个称为Ec 48的逆转子被激活，携带该逆转子元件的大肠杆菌死亡，感染性噬菌体丢失。借助数学模型，我们探讨了先验条件下，逆转录病毒将保护细菌种群免受噬菌体的捕食和条件下，逆转录病毒携带细菌将在没有这个元素的种群中进化。利用等基因E. coli中加入和不加入Ec 48和λVIR，我们估计了我们模型的参数，并检验了我们对其性质分析所产生的假设。我们的模型和实验表明，表达逆转录病毒介导的流产感染系统的细胞可以保护细菌种群。我们的结果表明，携带retron的细菌仅在有限的条件下具有竞争优势。
Retrons were described in 1984 as DNA sequences that code for a reverse transcriptase and a unique single-stranded DNA/RNA hybrid called multicopy single-stranded DNA (msDNA). It would not be until 2020 that a function was shown for retrons, when compelling evidence was presented that retrons activate an abortive infection pathway in response to bacteriophage (phage) infection. When infected with the virulent mutant of the phage lambda, λVIR, and to a lesser extent, other phages, a retron designated Ec48 is activated, the Escherichia coli bearing this retron element dies, and the infecting phage is lost. With the aid of a mathematical model, we explore the a priori conditions under which retrons will protect bacterial populations from predation by phage and the conditions under which retron-bearing bacteria will evolve in populations without this element. Using isogenic E. coli with and without Ec48 and λVIR, we estimated the parameters of our model and tested the hypotheses generated from our analysis of its properties. Our models and experiments demonstrate that cells expressing a retron-mediated abortive infection system can protect bacterial populations. Our results demonstrate that retron bearing bacteria only have a competitive advantage under a limited set of conditions.
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影响因子：
14.9
作者：
Simon DM;Zimmerly S
通讯作者：
Zimmerly S
DOI：
10.1073/pnas.78.10.6324
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CHAO, L;LEVIN, BR
通讯作者：
LEVIN, BR
影响因子：
2.9
作者：
STEWART, FM;LEVIN, BR
通讯作者：
LEVIN, BR
影响因子：
14.9
作者：
Simon, Anna J.;Ellington, Andrew D.;Finkelstein, Ilya J.
通讯作者：
Finkelstein, Ilya J.
影响因子：
4.6
作者：
Fukuyo, Masaki;Sasaki, Akira;Kobayashi, Ichizo
通讯作者：
Kobayashi, Ichizo