Bactericidal Effect of Pseudomonas oryziphila sp. nov., a Novel Pseudomonas Species Against Xanthomonas oryzae Reduces Disease Severity of Bacterial Leaf Streak of Rice.
Bactericidal Effect of Pseudomonas oryziphila sp. nov., a Novel Pseudomonas Species Against Xanthomonas oryzae Reduces Disease Severity of Bacterial Leaf Streak of Rice.
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米假单胞菌的杀菌作用。
DOI：
10.3389/fmicb.2021.759536
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发表时间：
2021
影响因子：
5.2
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Yang R;Li S;Li Y;Yan Y;Fang Y;Zou L;Chen G
假单胞菌是γ-变形菌纲中一个多样的属，越来越多的新种表现出多种特性，包括抗菌和杀虫活性以及促进植物生长的特性，这使它们非常适合作为某些病原体的生物防治剂。在此我们分离出菌株1257，它对水稻黄单胞菌的两个致病变种表现出强烈的拮抗活性，尤其是对引起水稻细菌性条斑病（BLS）的水稻黄单胞菌水稻致病变种（Xoc）。系统发育、基因组、生理和生化特征表明菌株1257是一个新的假单胞菌物种的代表，它与昆虫病原细菌嗜虫假单胞菌关系最为密切。我们提议将其命名为嗜稻假单胞菌新种（Pseudomonas oryziphila sp. nov.）。比较基因组分析表明，嗜稻假单胞菌1257拥有两个密切相关的菌株——嗜虫假单胞菌L48和莫塞尔假单胞菌CFML 90 - 83的大部分核心代谢基因，以及一组编码Ⅳ型菌毛系统的基因，这表明它具有多样的代谢和运动特性。一些特性，如杀虫毒素、磷酸盐溶解、吲哚 - 3 - 乙酸和苯乙酸降解等被揭示出来。全基因组随机诱变显示，由LgrD催化的非核糖体肽可能是嗜稻假单胞菌1257针对Xoc RS105的一种主要活性化合物，以及氨甲酰磷酸和戊糖磷酸途径在控制该目标化合物生物合成中起关键作用。我们的研究结果表明，1257能够有效抑制Xoc在水稻组织中的生长和迁移，从而预防BLS疾病。据我们所知，这是首次报道一个对Xoc表现出强烈抗菌活性的新假单胞菌物种。结果表明，嗜稻假单胞菌菌株可能是一种有前景的BLS生物防治剂。
Pseudomonas is a diverse genus of Gammaproteobacteria with increasing novel species exhibiting versatile trains including antimicrobial and insecticidal activity, as well as plant growth–promoting, which make them well suited as biocontrol agents of some pathogens. Here we isolated strain 1257 that exhibited strong antagonistic activity against two pathovars of Xanthomonas oryzae, especially X. oryzae pv. oryzicola (Xoc) responsible for the bacterial leaf streak (BLS) in rice. The phylogenetic, genomic, physiological, and biochemical characteristics support that strain 1257 is a representative of a novel Pseudomonas species that is most closely related to the entomopathogenic bacterium Pseudomonas entomophila. We propose to name it Pseudomonas oryziphila sp. nov. Comparative genomics analyses showed that P. oryziphila 1257 possesses most of the central metabolic genes of two closely related strains P. entomophila L48 and Pseudomonas mosselii CFML 90-83, as well as a set of genes encoding the type IV pilus system, suggesting its versatile metabolism and motility properties. Some features, such as insecticidal toxins, phosphate solubilization, indole-3-acetic acid, and phenylacetic acid degradation, were disclosed. Genome-wide random mutagenesis revealed that the non-ribosomal peptide catalyzed by LgrD may be a major active compound of P. oryziphila 1257 against Xoc RS105, as well as the critical role of the carbamoyl phosphate and the pentose phosphate pathway that control the biosynthesis of this target compound. Our findings demonstrate that 1257 could effectively inhibit the growth and migration of Xoc in rice tissue to prevent the BLS disease. To our knowledge, this is the first report of a novel Pseudomonas species that displays a strong antibacterial activity against Xoc. The results suggest that the P. oryziphila strain could be a promising biological control agent for BLS.
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米黄单胞菌中国分离株转录激活因子样效应基因的遗传多样性。
影响因子：
3.2
作者：
Ji, Zhi-Yuan;Zakria, Muhammad;Chen, Gong-You
通讯作者：
Chen, Gong-You
影响因子：
3.2
作者：
Pan, Xiayan;Wu, Jian;Zhou, Mingguo
通讯作者：
Zhou, Mingguo
使用orthomcl将蛋白质分配给Orthomcl-DB组或将蛋白质组群集分为新的直系同源组。
影响因子：
--
作者：
Fischer, Steve;Brunk, Brian P;Chen, Feng;Gao, Xin;Harb, Omar S;Iodice, John B;Shanmugam, Dhanasekaran;Roos, David S;Stoeckert, Christian J Jr
通讯作者：
Stoeckert, Christian J Jr
对铜绿假单胞菌 PA1201 菌株的中央生物合成和次级代谢途径进行工程设计，以提高吩嗪-1-羧酸的产量
影响因子：
8.4
作者：
Jin, Kaiming;Zhou, Lian;He, Ya-Wen
通讯作者：
He, Ya-Wen
DOI：
10.1099/00207713-52-2-363
发表时间：
2002-03-01
影响因子：
2.8
作者：
Dabboussi, F;Hamze, M;Izard, D
通讯作者：
Izard, D