Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal
Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal
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雄性大鼠伏隔核、背侧海马和杏仁核对吗啡戒断引起的条件性地方厌恶的蛋白质组学分析
DOI：
10.1016/j.bbr.2019.112008
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发表时间：
2019-10
影响因子：
2.7
通讯作者：
Liu Jing gen
中科院分区：
文献类型：
--
作者：
Guo Liu bin;Yu Chuan;Ling Qing lan;Fu Yu;Wang Yu jun;Liu Jing gen
成瘾的特征是强迫性的药物寻求和服用行为，这被认为是由基因表达变化编码的持续神经适应造成的。我们之前证明了突触可塑性的变化是与吗啡戒断相关的厌恶记忆形成所必需的。然而，参与突触可塑性和厌恶记忆形成的蛋白质尚未得到很好的探索。在本研究中，我们采用基于二维凝胶电泳（2-DE）的蛋白质组学技术检测了条件吗啡戒断大鼠伏隔核、杏仁核和海马背侧蛋白表达的变化。我们发现，条件吗啡戒断后，杏仁核和海马背侧有23种蛋白发生显著改变。这些蛋白根据其生物学功能可分为能量代谢、信号转导、突触传递、细胞骨架蛋白、伴侣蛋白、蛋白质代谢等多种类型。western blot分析进一步证实了8个与突触可塑性相关的蛋白。很有可能这些鉴定的蛋白质可能有助于条件吗啡戒断诱导的神经可塑性和厌恶记忆的形成。因此，我们的工作将有助于理解与药物戒断记忆产生相关的潜在机制。
Addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations, encoded by changes of gene expression. We previously demonstrated that the changes in synaptic plasticity were required for the formation of aversive memories associated with morphine withdrawal. However, the proteins involved in synaptic plasticity and aversive memory formation have not been well explored. In the present study, we employed a two-dimensional gel electrophoresis (2-DE)-based proteomic technique to detect the changes of protein expression in the nucleus accumbens, amygdala and dorsal hippocampus of the rats that had developed conditioned morphine withdrawal. We found that twenty-three proteins were significantly altered in the amygdala and dorsal hippocampus after conditioned morphine withdrawal. These proteins can be classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and protein metabolism according to their biological functions. Eight proteins related to synaptic plasticity were further confirmed by western blot analysis. It is very likely that these identified proteins may contribute to conditioned morphine withdrawal-induced neural plasticity and aversive memory formation. Thus, our work will help understand the potential mechanism associated with generation of drug withdrawal memories.
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具有吗啡耐受性大鼠的脊髓蛋白的磷酸蛋白质组学和生物信息学分析。
影响因子：
3.7
作者：
Liaw WJ;Tsao CM;Huang GS;Wu CC;Ho ST;Wang JJ;Tao YX;Shui HA
通讯作者：
Shui HA
影响因子：
3.3
作者：
L. Stinus;M. Moal;G. Koob
通讯作者：
L. Stinus;M. Moal;G. Koob
DOI：
10.1590/s1679-45082014ao3042
发表时间：
2014-09
期刊：
Einstein (Sao Paulo, Brazil)
影响因子：
--
作者：
Moura AC;Lazzari VM;Agnes G;Almeida S;Giovenardi M;Veiga AB
通讯作者：
Veiga AB
影响因子：
3.5
作者：
Baghel, Meghraj Singh;Thakur, Mahendra Kumar
通讯作者：
Thakur, Mahendra Kumar
影响因子：
64.8
作者：
SOLLNER, T;WHITEHART, SW;ROTHMAN, JE
通讯作者：
ROTHMAN, JE