Metabolic status rather than cell cycle signals control quiescence entry and exit.
Metabolic status rather than cell cycle signals control quiescence entry and exit.
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DOI：
10.1083/jcb.201009028
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发表时间：
2011-03-21
期刊：
影响因子：
--
通讯作者：
Sagot I
中科院分区：
文献类型：
--
作者：
Laporte D;Lebaudy A;Sahin A;Pinson B;Ceschin J;Daignan-Fornier B;Sagot I
新的候选标记酵母静止的使用表明，静止的进入和退出主要依赖于细胞代谢状态，可以从细胞周期解偶联。静止被定义为增殖的暂时停滞，但它可能包括各种细胞情况。我们对这种广泛存在的细胞状态的了解仍然有限。特别是，人们对协调静止期建立和退出的分子决定因素知之甚少。在这里，我们表明，在碳源耗尽，芽殖酵母可以进入静止从所有细胞周期阶段。此外，使用细胞结构的候选标记静止，我们发现，静止退出的第一步可以触发独立的细胞生长和增殖的唯一除了葡萄糖在酿酒酵母和裂殖酵母粟酒裂殖酵母。重要的是，葡萄糖需要被内化和分解代谢，一直到糖酵解，以动员静止的细胞特定结构，但引人注目的是，ATP补充显然不是关键信号。总而言之，这些发现强烈地表明，静止期的进入和退出主要依赖于细胞代谢状态，并且可以与细胞周期解偶联。
The use of new candidate markers for yeast quiescence reveals that quiescence entry and exit primarily rely on cellular metabolic status and can be uncoupled from the cell cycle. Quiescence is defined as a temporary arrest of proliferation, yet it likely encompasses various cellular situations. Our knowledge about this widespread cellular state remains limited. In particular, little is known about the molecular determinants that orchestrate quiescence establishment and exit. Here we show that upon carbon source exhaustion, budding yeast can enter quiescence from all cell cycle phases. Moreover, using cellular structures that are candidate markers for quiescence, we found that the first steps of quiescence exit can be triggered independently of cell growth and proliferation by the sole addition of glucose in both Saccharomyces cerevisiae and Schizosaccharomyces pombe. Importantly, glucose needs to be internalized and catabolized all the way down to glycolysis to mobilize quiescent cell specific structures, but, strikingly, ATP replenishment is apparently not the key signal. Altogether, these findings strongly suggest that quiescence entry and exit primarily rely on cellular metabolic status and can be uncoupled from the cell cycle.
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影响因子：
9.8
作者：
Coller HA;Sang L;Roberts JM
通讯作者：
Roberts JM
影响因子：
2.9
作者：
Sambasivan, Ramkumar;Pavlath, Grace K.;Dhawan, Jyotsna
通讯作者：
Dhawan, Jyotsna
影响因子：
2.1
作者：
Shimanuki, Mizuki;Chung, Soo-Yoel;Yanagida, Mitsuhiro
通讯作者：
Yanagida, Mitsuhiro
DOI：
10.1083/jcb.200604072
发表时间：
2006-07-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Allen C;Büttner S;Aragon AD;Thomas JA;Meirelles O;Jaetao JE;Benn D;Ruby SW;Veenhuis M;Madeo F;Werner-Washburne M
通讯作者：
Werner-Washburne M
影响因子：
56.9
作者：
HARTWELL, LH;CULOTTI, J;REID, BJ
通讯作者：
REID, BJ