Combined optical coherence tomography and light sheet fluorescence microscopy for embryonic imaging
Combined optical coherence tomography and light sheet fluorescence microscopy for embryonic imaging
复制标题
结合光学相干断层扫描和光片荧光显微镜进行胚胎成像
DOI：
10.1117/12.2610091
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Larin, Kirill V.
中科院分区：
文献类型：
--
作者：
Karim, Md Mobarak;Sun, Ruijiao;Khajavi, Behzad;Singh, Manmohan;Chawla, Harshdeep S.;Ambekar, Yogeshwari S.;Schill, Alexander W.;Mayerich, David;Dickinson, Mary E.;Larin, Kirill V.
光学相干层析成像(OCT)和薄层荧光显微镜(LSFM)是发育生物学(如胚胎成像)中常用的成像技术。然而，每一种技术都有自己的缺点，如OCT和视野(FOV)的分辨率和分子专一性以及LSFM的速度。为了克服小动物胚胎成像的这些局限性，我们开发了一种结合OCT和LSFM的共对准多模式成像系统。OCT探头和LSFM激励光束组合在一起，通过相同的物镜用振镜进行扫描。光片厚度约13μm。激光扫描显微镜采集臂由0.8数值孔径水浸物镜、管状透镜和ccd摄像机组成，横向分辨率为~2.1μm。OCT系统基于中心波长为1050 nm、横向分辨率为~15µm、轴向分辨率为~7μm的100 kHz扫描源激光器。由于OCT系统和LSFM系统是共同对准的，因此图像配准简单明了，无需复杂的配准技术即可实现高通量多模式成像。
Optical coherence tomography (OCT) and light sheet fluorescence microscopy (LSFM) are well-established imaging techniques preferred in developmental biology, e.g., embryonic imaging. However, each technique has its own drawbacks, such as resolution and molecular specificity with OCT and field-of-view (FOV) and speed with LSFM. To overcome these limitations for small animal embryo imaging, we have developed a co-aligned multimodal imaging system combining OCT and LSFM. The OCT probe and LSFM excitation beams were combined and scanned with a galvanometer-mounted mirror through the same objective lens. The light sheet thickness was ~13 μm. The LSFM collection arm consisted of a 0.8 numerical aperture water immersion objective, tube lens, and CCD camera, resulting in a transverse resolution of ~2.1 μm. The OCT system was based on a 100 kHz swept-source laser with a central wavelength of 1050 nm and had a lateral resolution of ~15 µm and an axial resolution of ~7 μm. Images of fluorescent microbeads and a fluorescent-tagged mouse embryo at gestational day 9.5 showed the capabilities of the multimodal imaging system. Since the OCT system and LSFM system were co-aligned, image registration was straightforward and enabled high-throughput multimodal imaging without the need for complex registration techniques.
影响因子：
4.4
作者：
Fujimoto J;Swanson E
通讯作者：
Swanson E
影响因子：
3.6
作者：
Wang S;Singh M;Lopez AL 3rd;Wu C;Raghunathan R;Schill A;Li J;Larin KV;Larina IV
通讯作者：
Larina IV