Transcriptomic analysis for genetic mechanisms of the factors related to biofilm formation in Escherichia coli O157:H7.
Transcriptomic analysis for genetic mechanisms of the factors related to biofilm formation in Escherichia coli O157:H7.
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DOI：
10.1007/s00284-010-9862-4
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发表时间：
2011-04
影响因子：
2.6
通讯作者：
Lee, Jintae
中科院分区：
文献类型：
--
作者：
Lee, Jin-Hyung;Kim, Yong-Guy;Cho, Moo Hwan;Wood, Thomas K.;Lee, Jintae
对肠出血性大肠杆菌O157：H7(EDL933，STX1+和Stx2+)和86-24(Stx2+)两个谱系进行了研究，以确定非生物表面生物膜形成的遗传学基础。菌株EDL933在聚苯乙烯平板和聚乙烯管上几乎没有形成生物膜，而菌株86-24形成了坚固的生物膜。EDL933与86-24的全转录组图谱表明，在强生物膜形成菌株中，参与卷曲生物合成(csgBAC和csgDEFG)和纤维素产生(Adra)的基因显著诱导，而参与吲哚信号转导的基因(trpLED和mtr)受到最大程度的抑制。此外，49个噬菌体基因在两个菌株之间被高度诱导和抑制。使用刚果红平板和扫描电子显微镜进行的卷曲检测证实了微阵列数据，因为EDL933菌株产生了大量的卷曲，而菌株86-24形成的卷曲要少得多。此外，EDL933产生的纤维素量是86-24的19倍，而吲哚的产量是86-24的两倍。因此，看来大肠杆菌O157：H7 EDL933产生更多的生物膜，因为它增加了卷曲和纤维素的产生，减少了吲哚的产生。
Two lineages of enterohemorrhagic Escherichia coli O157:H7 (EDL933, Stx1+ and Stx2+) and 86-24 (Stx2+) were investigated to determine the genetic basis of biofilm formation on abiotic surfaces. Strain EDL933 formed a robust biofilm while strain 86-24 formed almost no biofilm on either polystyrene plates or polyethylene tubes. Whole-transcriptome profiles of EDL933 vs. 86-24 revealed that in the strong biofilm-forming strain, genes involved in curli biosynthesis (csgBAC and csgDEFG) and cellulose production (adrA) were significantly induced, whereas genes involved in indole signaling (trpLED and mtr) were most repressed. Additionally, 49 phage genes were highly induced and repressed between the two strains. Curli assays using Congo red plates and scanning electron microscopy corroborated the microarray data as the EDL933 strain produced a large amount of curli, while strain 86-24 formed much less curli. Moreover, EDL933 produced 19-fold more cellulose than 86-24, and indole production in EDL933 was two times lower than that of the strain 86-24. Therefore, it appears E. coli O157:H7 EDL933 produces more biofilm because of its increased curli and cellulose production and reduced indole production.
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影响因子：
4.2
作者：
Dowd SE;Ishizaki H
通讯作者：
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影响因子：
64.8
作者：
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11.8
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作者：
Lee, Jintae;Bansal, Tarun;Wood, Thomas K.
通讯作者：
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