Pediatric tubular pulmonary heart valve from decellularized engineered tissue tubes.
Pediatric tubular pulmonary heart valve from decellularized engineered tissue tubes.
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DOI：
10.1016/j.biomaterials.2015.05.009
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发表时间：
2015-09
期刊：
影响因子：
14
通讯作者：
Tranquillo, Robert T.
中科院分区：
文献类型：
--
作者：
Reimer, Jay M.;Syedain, Zeeshan H.;Haynie, Bee H. T.;Tranquillo, Robert T.
儿科患者占进行的心脏瓣膜置换术的一小部分，但具有生长潜力的儿科肺动脉瓣置换术仍然是未满足的临床需求。在这里，我们报告了第一个管状心脏瓣膜，由两个脱细胞的，工程化的组织管连接可吸收缝线，这可以满足这一需求，原则上。通过使绵羊真皮成纤维细胞用对齐的细胞产生的胶原基质替换牺牲纤维蛋白凝胶，随后将其脱细胞，来制造工程化组织管。以前，这些工程管在植入羊股动脉后被广泛再细胞化。因此，由这些管制成的管状瓣膜可适于再细胞化，并且理想地适于体细胞生长。根据管状瓣膜设计，缝合线图案在内管中产生三个等间距的“瓣叶”，当暴露于背压时向内塌陷。在配备有二次流动回路的脉冲复制器系统中进行瓣膜测试，以允许根部扩张。所有组织工程瓣膜在肺动脉条件下均表现出瓣叶完全打开和关闭、最小返流（< 5%）和低收缩压梯度（< 2.5 mmHg）。在各种跨根部压差下保持瓣膜性能，并且在200万次疲劳试验循环后没有明显的组织损伤。
Pediatric patients account for a small portion of the heart valve replacements performed, but a pediatric pulmonary valve replacement with growth potential remains an unmet clinical need. Herein we report the first tubular heart valve made from two decellularized, engineered tissue tubes attached with absorbable sutures, which can meet this need, in principle. Engineered tissue tubes were fabricated by allowing ovine dermal fibroblasts to replace a sacrificial fibrin gel with an aligned, cell-produced collagenous matrix, which was subsequently decellularized. Previously, these engineered tubes became extensively recellularized following implantation into the sheep femoral artery. Thus, a tubular valve made from these tubes may be amenable to recellularization and, ideally, somatic growth. The suture line pattern generated three equi-spaced “leaflets” in the inner tube, which collapsed inward when exposed to back pressure, per tubular valve design. Valve testing was performed in a pulse duplicator system equipped with a secondary flow loop to allow for root distention. All tissue-engineered valves exhibited full leaflet opening and closing, minimal regurgitation (< 5%), and low systolic pressure gradients (< 2.5 mmHg) under pulmonary conditions. Valve performance was maintained under various trans-root pressure gradients and no tissue damage was evident after 2 million cycles of fatigue testing.
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DOI：
10.1007/bf00266843
发表时间：
1989-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
DAYAN, D;HISS, Y;WOLMAN, M
通讯作者：
WOLMAN, M
影响因子：
14
作者：
Syedain, Zeeshan H.;Tranquillo, Robert T.
通讯作者：
Tranquillo, Robert T.
影响因子：
4.1
作者：
Syedain, Zeeshan H.;Meier, Lee A.;Tranquillo, Robert T.
通讯作者：
Tranquillo, Robert T.
影响因子：
4.1
作者：
Syedain, Zeeshan H.;Bradee, Allison R.;Tranquillo, Robert T.
通讯作者：
Tranquillo, Robert T.
影响因子：
14
作者：
Weber, Benedikt;Dijkman, Petra E.;Hoerstrup, Simon P.
通讯作者：
Hoerstrup, Simon P.