Caenorhabditis elegans battling starvation stress: low levels of ethanol prolong lifespan in L1 larvae.
Caenorhabditis elegans battling starvation stress: low levels of ethanol prolong lifespan in L1 larvae.
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DOI：
10.1371/journal.pone.0029984
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Clarke SG
中科院分区：
文献类型：
--
作者：
Castro PV;Khare S;Young BD;Clarke SG
线虫秀丽隐杆线虫在孵化时没有食物的情况下，会在第一个幼虫阶段停止发育。这一阶段的幼虫为研究发育过程中的应激反应提供了一个很好的模型。我们发现，补充饥饿的幼虫与乙醇显着延长其寿命在此L1滞育。乙醇诱导的寿命延长的效果，可以观察到当乙醇被添加到培养基中在孵化后0和10天之间的任何时间。延长寿命的最低乙醇浓度为1 mM（0.005%）;更高浓度至68 mM（0.4%）并不导致存活率增加。尽管它们的存活时间延长，但幼虫没有进展到L2阶段。用正丙醇和正丁醇补充饥饿的培养物也延长了寿命，但甲醇和异丙醇没有可测量的效果。线虫脂肪酸和氨基酸的质谱分析表明，L1幼虫可以将原子从乙醇到这两种类型的分子。基于这些数据，我们认为，乙醇补充剂可以延长寿命的L1幼虫作为一个碳源和能源和/或诱导应激反应。
The nematode Caenorhabditis elegans arrests development at the first larval stage if food is not present upon hatching. Larvae in this stage provide an excellent model for studying stress responses during development. We found that supplementing starved larvae with ethanol markedly extends their lifespan within this L1 diapause. The effects of ethanol-induced lifespan extension can be observed when the ethanol is added to the medium at any time between 0 and 10 days after hatching. The lowest ethanol concentration that extended lifespan was 1 mM (0.005%); higher concentrations to 68 mM (0.4%) did not result in increased survival. In spite of their extended survival, larvae did not progress to the L2 stage. Supplementing starved cultures with n-propanol and n-butanol also extended lifespan, but methanol and isopropanol had no measurable effect. Mass spectrometry analysis of nematode fatty acids and amino acids revealed that L1 larvae can incorporate atoms from ethanol into both types of molecules. Based on these data, we suggest that ethanol supplementation may extend the lifespan of L1 larvae by either serving as a carbon and energy source and/or by inducing a stress response.
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DOI：
10.1111/j.1530-0277.2008.00639.x
发表时间：
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影响因子：
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作者：
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