Interfamily transfer of Arabidopsis lectin-mediated antiviral gene confers resistance to pepino mosaic virus in tomato
Interfamily transfer of Arabidopsis lectin-mediated antiviral gene confers resistance to pepino mosaic virus in tomato
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拟南芥凝集素介导的抗病毒基因的科间转移赋予番茄对果皮花叶病毒的抗性
DOI：
10.1007/s10327-020-00917-3
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发表时间：
2020
影响因子：
1.2
通讯作者：
Yamaji Y.
中科院分区：
文献类型：
--
作者：
Okano Y.;Maejima K.;Yoshida T.;Nishida S.;Tokuda R.;Nishikawa M.;Namba S.;Yamaji Y.
在植物抗病毒育种中，编码核苷酸结合位点和富含亮氨酸重复序列（NB-LRR）蛋白的R基因是将抗病毒性状导入作物品种的主要遗传资源。然而，NB-LRR基因的科间转移-在属于与转移基因的来源植物在分类学上不同的科的植物中异源表达是困难的，因为它经常导致不适当的自身免疫，导致植物生长抑制或缺乏抗性。我们以前从拟南芥（拟南芥科）中鉴定了一个非NB-LRR型的抗马铃薯病毒基因JAX 1（JACALIN-TYPELECTINREQUIREDEFORPOTEXVIRUSRESISTANCE 1）。本研究利用瞬时表达技术证明了JAX 1基因对温室番茄生产中的马铃薯花叶病毒（PepMV）具有广泛的抗性，转JAX 1基因的本氏烟草（Nicotiana benthamiana）和茄科番茄植株对多种PepMV分离物表现出广泛和高水平的抗性。我们的研究结果表明，非NB-LRR型抗病基因，特别是凝集素家族的基因，将是有用的科间转移，并为生产抗病毒作物品种的有希望的遗传资源。
In plant antiviral breeding,Rgenes encoding nucleotide-binding site and leucine-rich repeat (NB-LRR) proteins serve as major genetic resources to introduce antiviral traits to crop cultivars. However, interfamily transfer—heterologous expression in a plant belonging to a taxonomically different family from the source plant of the transferred gene—of an NB-LRR gene is difficult because it often leads to inappropriate autoimmunity that results in inhibition of plant growth or lack of resistance. We previously identified a non-NB-LRR-type resistance gene,JACALIN-TYPE LECTIN REQUIRED FOR POTEXVIRUS RESISTANCE1(JAX1), fromArabidopsis thaliana(Brassicaceae). Here, we used a transient expression assay to demonstrate thatJAX1confers broad resistance to potexviruses including pepino mosaic virus (PepMV), which causes severe damage to greenhouse tomato production worldwide.JAX1-transgenicNicotiana benthamianaand tomato plants in theSolanaceaefamily exhibited broad and a high level resistance to multiple PepMV isolates. Our results suggest that non-NB-LRR-type resistance genes, especially lectin-family genes, will be useful for interfamily transfer and are promising genetic resources for producing virus-resistant crop cultivars.
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影响因子：
2.7
作者：
Hanssen, I. M.;Paeleman, A.;Thomma, B. P. H. J.
通讯作者：
Thomma, B. P. H. J.
影响因子：
3.5
作者：
Candresse, Thierry;Marais, Armelle;Bendahmane, Abdelhafid
通讯作者：
Bendahmane, Abdelhafid
DOI：
--
发表时间：
2017
期刊：
--
影响因子：
--
作者：
Steven;-A.;Whitham;Miki;-L.;Yamamoto;James;-C.;Carrington
通讯作者：
Steven;-A.;Whitham;Miki;-L.;Yamamoto;James;-C.;Carrington
影响因子：
7.2
作者：
Earley, KW;Haag, JR;Pikaard, CS
通讯作者：
Pikaard, CS
影响因子：
5.4
作者：
Tetsuya Yoshida;Takuya Shiraishi;Yuka Hagiwara-Komoda;K. Komatsu;K. Maejima;Yukari Okano;Yuji Fujimoto;Akira Yusa;Y. Yamaji;S. Namba
通讯作者：
Tetsuya Yoshida;Takuya Shiraishi;Yuka Hagiwara-Komoda;K. Komatsu;K. Maejima;Yukari Okano;Yuji Fujimoto;Akira Yusa;Y. Yamaji;S. Namba