Receptor for hyaluronan-mediated motility and CD44 expressions in colon cancer assessed by quantitative analysis using real-time reverse transcriptase-polymerase chain reaction.
Receptor for hyaluronan-mediated motility and CD44 expressions in colon cancer assessed by quantitative analysis using real-time reverse transcriptase-polymerase chain reaction.
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DOI：
10.1111/j.1349-7006.1999.tb00846.x
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发表时间：
1999-09
期刊：
影响因子：
--
通讯作者：
Kimata K
中科院分区：
文献类型：
--
作者：
Yamada Y;Itano N;Narimatsu H;Kudo T;Hirohashi S;Ochiai A;Niimi A;Ueda M;Kimata K
透明质酸（HA）介导的运动受体（RHAMM）是HA介导的运动受体，其表达与ras转化细胞的恶性相关，因为HA与该受体的结合激活了其迁移能力。HA的细胞表面受体CD 44也与一些癌细胞的转移行为有关。在这项研究中，我们使用真实的时间逆转录酶-聚合酶链反应方法在30例不同诊断阶段的结肠癌标本中检测了癌症进展与RHAMM、CD 44（所有形式）和CD 44外显子6的mRNA水平的关系。与癌旁组织相比，29例（97%）RHAMM、21例（70%）CD 44 s和19例（63%）外显子6的mRNA表达均增高。发现RHAMM表达与Dukes'分期A、B和C中的任何癌标本之间存在统计学显著相关性，并且在Dukes'分期C的标本中证实了CD 44 mRNA的过表达。因此，我们目前的研究首次表明，RHAMM表达可能是一个临床有用的指标结肠癌。
Receptor for hyaluronan (HA)‐mediated motility (RHAMM) is a receptor for HA‐mediated motility and its expression is correlated with malignancy of ras‐transformed cells in that binding of HA to this receptor activates their migratory ability. CD44, a cell surface receptor for HA is also implicated in metastatic behavior of some cancer cells. In this study we examined the relationships of cancer progression with mRNA levels of RHAMM, CD44 (all forms), and exon 6 of CD44 using the real‐time reverse transcriptase‐polymerase chain reaction method in specimens of colon cancers at different diagnostic stages from 30 patients. Increased mRNA levels of RHAMM were observed in 29 specimens (97%), CD44s (all forms) in 21 specimens (70%), and its exon 6 in 19 specimens (63%) in comparison with those in the corresponding noncancerous tissue specimens. A statistically significant correlation between RHAMM expression and cancerous specimens at any of Dukes' stages A, B, and C was found, and the overexpression of CD44 mRNAs was confirmed in specimens at Dukes' stage C. Thus, our present study for the first time suggests that RHAMM expression may be a clinically useful indicator of colon cancer.
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