Comparative Genomics of Disease and Carriage Serotype 1 Pneumococci.
Comparative Genomics of Disease and Carriage Serotype 1 Pneumococci.
复制标题
DOI：
10.1093/gbe/evac052
复制
发表时间：
2022-04-10
影响因子：
3.3
通讯作者：
Bentley, Stephen D.
中科院分区：
文献类型：
--
作者：
Chaguza, Chrispin;Ebruke, Chinelo;Senghore, Madikay;Lo, Stephanie W.;Tientcheu, Peggy-Estelle;Gladstone, Rebecca A.;Tonkin-Hill, Gerry;Cornick, Jennifer E.;Yang, Marie;Worwui, Archibald;McGee, Lesley;Breiman, Robert F.;Klugman, Keith P.;Kadioglu, Aras;Everett, Dean B.;Mackenzie, Grant;Croucher, Nicholas J.;Roca, Anna;Kwambana-Adams, Brenda A.;Antonio, Martin;Bentley, Stephen D.
关键词：
侵袭性疾病患者全身组织中肺炎链球菌血清型的分离与无症状携带者鼻咽组织中肺炎链球菌血清型的分离差异很大。一些血清型是高度侵袭性的，特别是1型，但由于携带型分离物的稀有，其潜在的遗传学仍然知之甚少，降低了与侵袭性分离物比较的能力。在这里，我们使用一项控制良好的全基因组关联研究来寻找与非洲1型血清流行环境中1型肺炎球菌侵袭性相关的遗传变异。我们没有发现一致的证据表明，侵袭性疾病患者的分离株中某些基因组变异的比例高于无症状携带者。总体而言，基因组变异解释了可以忽略的表型变异，表明对疾病状态的影响微乎其微。此外，随着时间的推移，谱系分布发生了变化，谱系相互取代，突显了持续病原体监测的重要性。我们的发现表明，超侵袭性是1型血清菌株的固有属性，并不是针对具有独特基因组变异的“疾病相关”亚群。
The isolation of Streptococcus pneumoniae serotypes in systemic tissues of patients with invasive disease versus the nasopharynx of healthy individuals with asymptomatic carriage varies widely. Some serotypes are hyper-invasive, particularly serotype 1, but the underlying genetics remain poorly understood due to the rarity of carriage isolates, reducing the power of comparison with invasive isolates. Here, we use a well-controlled genome-wide association study to search for genetic variation associated with invasiveness of serotype 1 pneumococci from a serotype 1 endemic setting in Africa. We found no consensus evidence that certain genomic variation is overrepresented among isolates from patients with invasive disease than asymptomatic carriage. Overall, the genomic variation explained negligible phenotypic variability, suggesting a minimal effect on the disease status. Furthermore, changes in lineage distribution were seen with lineages replacing each other over time, highlighting the importance of continued pathogen surveillance. Our findings suggest that the hyper-invasiveness is an intrinsic property of the serotype 1 strains, not specific for a “disease-associated” subpopulation disproportionately harboring unique genomic variation.
登录
查看更多内容
影响因子：
4.5
作者：
Chewapreecha C;Marttinen P;Croucher NJ;Salter SJ;Harris SR;Mather AE;Hanage WP;Goldblatt D;Nosten FH;Turner C;Turner P;Bentley SD;Parkhill J
通讯作者：
Parkhill J
影响因子：
2.7
作者：
Ebruke C;Roca A;Egere U;Darboe O;Hill PC;Greenwood B;Wren BW;Adegbola RA;Antonio M
通讯作者：
Antonio M
影响因子：
9.4
作者：
du Plessis M;Allam M;Tempia S;Wolter N;de Gouveia L;von Mollendorf C;Jolley KA;Mbelle N;Wadula J;Cornick JE;Everett DB;McGee L;Breiman RF;Gladstone RA;Bentley SD;Klugman KP;von Gottberg A
通讯作者：
von Gottberg A
影响因子：
9.4
作者：
Brueggemann, AB;Spratt, BG
通讯作者：
Spratt, BG
影响因子：
12.3
作者：
Brynildsrud O;Bohlin J;Scheffer L;Eldholm V
通讯作者：
Eldholm V