Detailed expression pattern of aldolase C (Aldoc) in the cerebellum, retina and other areas of the CNS studied in Aldoc-Venus knock-in mice.
Detailed expression pattern of aldolase C (Aldoc) in the cerebellum, retina and other areas of the CNS studied in Aldoc-Venus knock-in mice.
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DOI：
10.1371/journal.pone.0086679
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sugihara I
中科院分区：
文献类型：
--
作者：
Fujita H;Aoki H;Ajioka I;Yamazaki M;Abe M;Oh-Nishi A;Sakimura K;Sugihara I
醛缩酶C （Aldoc，也被称为“zebrin II”）是一种糖酵解酶的脑型同工酶，在小脑浦肯野细胞（PCs）亚群中表达异质性，这些细胞在小脑皮层以复杂的条纹模式纵向排列，这种模式与输入和输出轴突投影的地形密切相关。在这里，我们产生了敲入Aldoc-Venus小鼠，其中Aldoc的表达通过荧光蛋白Venus的表达来可视化。由于突变体在一般脑形态和小脑的条纹模式上没有明显的表型，我们利用Aldoc-Venus杂合子中的Venus表达来详细观察Aldoc在神经系统中的表达模式。Venus在小脑PCs、耳蜗背核侧轮细胞、内耳感觉上皮和所有主要类型的视网膜细胞中均有高水平表达，而在背根神经节星形胶质细胞和卫星细胞中均有中等水平表达。通过连续切片排列分析，仔细追踪不同程度表达金星的pc的条纹排列，并将其映射到整个小脑皮层的展开方案上，以重新识别所有单个Aldoc条纹。Aldoc表达的纵向条纹边界首次在小鼠小叶中被发现，并与攀爬纤维投射模式和另一种区室标记分子热休克蛋白25 （HSP25）的表达相关。与大鼠一样，小鼠的小脑核通过aldoc阳性和阴性PC轴突的明显突起分为背侧阴性和尾侧阳性部分。本研究中小脑Aldoc条纹的鉴定，如样品冠状和水平切片以及样品表面照片，可以在以后的实验中参考。
Aldolase C (Aldoc, also known as “zebrin II”), a brain type isozyme of a glycolysis enzyme, is expressed heterogeneously in subpopulations of cerebellar Purkinje cells (PCs) that are arranged longitudinally in a complex striped pattern in the cerebellar cortex, a pattern which is closely related to the topography of input and output axonal projections. Here, we generated knock-in Aldoc-Venus mice in which Aldoc expression is visualized by expression of a fluorescent protein, Venus. Since there was no obvious phenotypes in general brain morphology and in the striped pattern of the cerebellum in mutants, we made detailed observation of Aldoc expression pattern in the nervous system by using Venus expression in Aldoc-Venus heterozygotes. High levels of Venus expression were observed in cerebellar PCs, cartwheel cells in the dorsal cochlear nucleus, sensory epithelium of the inner ear and in all major types of retinal cells, while moderate levels of Venus expression were observed in astrocytes and satellite cells in the dorsal root ganglion. The striped arrangement of PCs that express Venus to different degrees was carefully traced with serial section alignment analysis and mapped on the unfolded scheme of the entire cerebellar cortex to re-identify all individual Aldoc stripes. A longitudinally striped boundary of Aldoc expression was first identified in the mouse flocculus, and was correlated with the climbing fiber projection pattern and expression of another compartmental marker molecule, heat shock protein 25 (HSP25). As in the rat, the cerebellar nuclei were divided into the rostrodorsal negative and the caudoventral positive portions by distinct projections of Aldoc-positive and negative PC axons in the mouse. Identification of the cerebellar Aldoc stripes in this study, as indicated in sample coronal and horizontal sections as well as in sample surface photos of whole-mount preparations, can be referred to in future experiments.
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影响因子：
2.5
作者：
HAWKES, R;LECLERC, N
通讯作者：
LECLERC, N
影响因子：
46.9
作者：
Nagai, T;Ibata, K;Miyawaki, A
通讯作者：
Miyawaki, A
影响因子：
2.5
作者：
BROCHU, G;MALER, L;HAWKES, R
通讯作者：
HAWKES, R
影响因子：
3.3
作者：
Hallem, JS;Thompson, JH;Bower, JM
通讯作者：
Bower, JM
DOI：
10.1073/pnas.84.23.8692
发表时间：
1987-12-01
影响因子：
11.1
作者：
MUGNAINI, E;MORGAN, JI
通讯作者：
MORGAN, JI