Experimental acute myocardial infarction: telocytes involvement in neo-angiogenesis.
Experimental acute myocardial infarction: telocytes involvement in neo-angiogenesis.
复制标题
DOI：
10.1111/j.1582-4934.2011.01449.x
复制
发表时间：
2011-11
影响因子：
5.3
通讯作者：
Popescu LM
中科院分区：
文献类型：
--
作者：
Manole CG;Cismaşiu V;Gherghiceanu M;Popescu LM
本实验采用大鼠实验性心肌梗死模型，研究了梗死边缘区超微结构的恢复，特别是新生血管的形成。我们对端细胞（TC）的可能作用感兴趣，端细胞是最近在心肌中发现的一种新型间质细胞（见）。电子显微镜、免疫细胞化学和几种促血管生成microRNA的分析提供了TC参与心肌梗死后新血管生成的证据。电子显微镜观察显示TC与新生血管生成元素的密切空间关联。更高分辨率的图像提供了以下信息：（a）内皮细胞的近腔面与其周围TC之间的细胞间隙通常小于50 nm;（B）TC与内皮细胞建立多个直接纳米接触，其中细胞外间隙似乎被消除;此类纳米接触的长度为0.4-1.5 μm;（c）内皮细胞近腔面无基底膜。除了物理接触（纳米级或微观），TC可能通过旁分泌促进新血管生成（如VEGF或NOS 2的免疫细胞化学所示）。最后但并非最不重要的是，TC含有可测量数量的血管生成microRNA（例如let-7 e、10a、21、27 b、100、126- 3 p、130 a、143、155、503）。两者合计，直接（物理）接触的TC与内皮管，以及间接（化学）的积极影响内的“血管生成区”，表明了一个重要的参与TC在心肌梗死的晚期阶段的新血管生成。
We used rat experimental myocardial infarction to study the ultrastructural recovery, especially neo-angiogenesis in the infarction border zone. We were interested in the possible role(s) of telocytes (TCs), a novel type of interstitial cell very recently discovered in myocardim (see ). Electron microscopy, immunocytochemistry and analysis of several proangiogenic microRNAs provided evidence for TC involvement in neo-angiogenesis after myocardial infarction. Electron microscopy showed the close spatial association of TCs with neoangiogenetic elements. Higher resolution images provided the following information: (a) the intercellular space between the abluminal face of endothelium and its surrounding TCs is frequently less than 50 nm; (b) TCs establish multiple direct nanocontacts with endothelial cells, where the extracellular space seems obliterated; such nanocontacts have a length of 0.4–1.5 μm; (c) the absence of basal membrane on the abluminal face of endothelial cell. Besides the physical contacts (either nanoscopic or microscopic) TCs presumably contribute to neo-angiognesis via paracrine secretion (as shown by immunocytochemistry for VEGF or NOS2). Last but not least, TCs contain measurable quantities of angiogenic microRNAs (e.g. let-7e, 10a, 21, 27b, 100, 126-3p, 130a, 143, 155, 503). Taken together, the direct (physical) contact of TCs with endothelial tubes, as well as the indirect (chemical) positive influence within the ‘angiogenic zones’, suggests an important participation of TCs in neo-angiogenesis during the late stage of myocardial infarction.
登录
查看更多内容
影响因子：
3.6
作者：
Hinescu, Mihail E.;Gherghiceanu, Mihaela;Suciu, Laura;Popescu, Laurentiu M.
通讯作者：
Popescu, Laurentiu M.
影响因子：
5.3
作者：
Eyden B
通讯作者：
Eyden B
影响因子：
5.2
作者：
Li, Tao-Sheng;Cheng, Ke;Lee, Shuo-Tsan;Matsushita, Satoshi;Davis, Darryl;Malliaras, Konstantinos;Zhang, Yiqiang;Matsushita, Noriko;Smith, Rachel Ruckdeschel;Marban, Eduardo
通讯作者：
Marban, Eduardo
DOI：
10.1161/atvbaha.109.198895
发表时间：
2010-06-01
影响因子：
8.7
作者：
Kumar, Arun H. S.;Caplice, Noel M.
通讯作者：
Caplice, Noel M.
影响因子：
20.1
作者：
Kuehbacher, Angelika;Urbich, Carmen;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie