Comprehensive Quality Assessment Based Specific Chemical Profiles for Geographic and Tissue Variation in Gentiana rigescens Using HPLC and FTIR Method Combined with Principal Component Analysis.
Comprehensive Quality Assessment Based Specific Chemical Profiles for Geographic and Tissue Variation in Gentiana rigescens Using HPLC and FTIR Method Combined with Principal Component Analysis.
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DOI：
10.3389/fchem.2017.00125
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发表时间：
2017
影响因子：
5.5
通讯作者：
Wang YZ
中科院分区：
文献类型：
--
作者：
Li J;Zhang J;Zhao YL;Huang HY;Wang YZ
龙胆（Gentiana rigescens Franch.）采用HPLC-DAD和FTIR结合主成分分析（PCA）方法，对云南6个产地（n = 240）中龙胆苦苷、马钱酸、当药苷和当药苦苷的含量及化学成分进行了评价。龙胆苦苷（主要环烯醚萜苷）的含量在龙胆中最高。rigescens，无论组织和地理来源。昆明和曲靖样品中的当药苦苷含量最低，甚至未检出。龙胆苦苷、落干酸、当药苷和当药苦苷在组织间和组织内均存在显著的相关性（p < 0.05），且具有高度的地理来源依赖性，表明环境条件对龙胆次生代谢产物的转化和转运有影响。僵硬此外，还将样品合理地划分为沿着气候条件相似的大产区的3个聚类，其特征为碳水化合物、酚类、苯甲酸酯类、萜类、脂肪醇类、芳烃类等。本工作提供了全球范围内G. rigescens来源于6个不同的产地，这有助于系统地控制中药的质量。
Roots, stems, leaves, and flowers of Longdan (Gentiana rigescens Franch. ex Hemsl) were collected from six geographic origins of Yunnan Province (n = 240) to implement the quality assessment based on contents of gentiopicroside, loganic acid, sweroside and swertiamarin and chemical profile using HPLC-DAD and FTIR method combined with principal component analysis (PCA). The content of gentiopicroside (major iridoid glycoside) was the highest in G. rigescens, regardless of tissue and geographic origin. The level of swertiamarin was the lowest, even unable to be detected in samples from Kunming and Qujing. Significant correlations (p < 0.05) between gentiopicroside, loganic acid, sweroside, and swertiamarin were found at inter- or intra-tissues, which were highly depended on geographic origins, indicating the influence of environmental conditions on the conversion and transport of secondary metabolites in G. rigescens. Furthermore, samples were reasonably classified as three clusters along large producing areas where have similar climate conditions, characterized by carbohydrates, phenols, benzoates, terpenoids, aliphatic alcohols, aromatic hydrocarbons, and so forth. The present work provided global information on the chemical profile and contents of major iridoid glycosides in G. rigescens originated from six different origins, which is helpful for controlling quality of herbal medicines systematically.
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DOI：
10.1016/j.jpba.2010.09.030
发表时间：
2011-02-20
影响因子：
3.4
作者：
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