Downregulation of Long Noncoding RNA LUCAT1 Suppresses the Migration and Invasion of Bladder Cancer by Targeting miR-181c-5p.
Downregulation of Long Noncoding RNA LUCAT1 Suppresses the Migration and Invasion of Bladder Cancer by Targeting miR-181c-5p.
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DOI：
10.1155/2020/4817608
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发表时间：
2020
影响因子：
--
通讯作者：
Yao X
中科院分区：
文献类型：
--
作者：
Chen Y;Zhang W;Shen L;Kadier A;Huang J;Wang R;Wu P;Yao X
据报道，长链非编码RNA LUCAT1（肺癌相关转录物1）在膀胱癌样本中高表达，但其作用和分子机制尚待阐明。生物信息学方法表明miR-181c-5p是LUCAT1的靶标。在这里，我们旨在揭示LUCAT1是否通过靶向miR-181c-5p参与膀胱癌的发展。采用RT-PCR技术检测膀胱细胞和组织中LUCAT1和miR-181c-5p的表达水平。通过CCK-8、创面愈合、Transwell室和流式细胞术检测LUCAT1/miR-181c-5p轴对细胞增殖、迁移、侵袭和凋亡的影响。western blotting检测凋亡/迁移相关蛋白的表达。结果表明，LUCAT1在膀胱癌组织和细胞中过表达，而miR-181c-5p与正常膀胱细胞和组织相比表达水平较低。在UM-UC-3和T24细胞系中，LUCAT1沉默后，细胞增殖、迁移和侵袭能力明显受损，细胞凋亡增强，但miR-181c-5p下调可消除这种影响。此外，miR-181c-5p下调可导致LUCAT1下调，从而介导Bcl2和N-cadherin表达降低，Bax和E-cadherin表达升高。此外，我们发现KRAS是miR-181c-5p的直接靶点，并受到LUCAT1的正调控。综上所述，本研究揭示了LUCAT1的下调以mir -181c-5p依赖的方式抑制膀胱癌细胞的迁移和侵袭，这可能与KRAS下调有关。
The long noncoding RNA LUCAT1 (lung cancer-associated transcript 1) has been reported to be highly expressed in bladder cancer samples, but its role and molecular mechanisms need to be elucidated. Bioinformatics methods show that miR-181c-5p is a target of LUCAT1. Here, we aimed to reveal whether LUCAT1 participates in the development of bladder cancer via targeting miR-181c-5p. The expression levels of LUCAT1 and miR-181c-5p were detected by RT-PCR technology in bladder cells and tissues. The effects of the LUCAT1/miR-181c-5p axis on cell proliferation, migration, invasion, and apoptosis were tested by CCK-8, wound healing, Transwell chambers, and flow cytometry assays. The expressions of apoptosis/migration-related proteins were detected by western blotting assays. The results demonstrated that LUCAT1 was overexpressed in bladder cancer tissue and cells, while miR-181c-5p showed a low expression pattern as compared to normal bladder cells and tissues. Cell proliferation, migration, and invasion capacities were significantly impaired, and cell apoptosis was enhanced when LUCAT1 was silenced in UM-UC-3 and T24 cell lines, but this effect was abolished by miR-181c-5p downregulation. In addition, miR-181c-5p downregulation impaired LUCAT1 downregulation which mediated the decreased expressions of Bcl2 and N-cadherin and the increased expressions of Bax and E-cadherin. Moreover, we found that KRAS was a direct target of miR-181c-5p and was under the positive regulation of LUCAT1. Collectively, this study reveals that knockdown of LUCAT1 inhibits the migration and invasion of bladder cancer cells in a miR-181c-5p-dependent manner, which may be related to KRAS downregulation.
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Diana-LNCBASE：长期非编码RNA上经过实验验证和计算预测的microRNA靶标。
影响因子：
14.9
作者：
Paraskevopoulou MD;Georgakilas G;Kostoulas N;Reczko M;Maragkakis M;Dalamagas TM;Hatzigeorgiou AG
通讯作者：
Hatzigeorgiou AG
影响因子：
3.7
作者：
Sanchez-Diaz PC;Hsiao TH;Chang JC;Yue D;Tan MC;Chen HI;Tomlinson GE;Huang Y;Chen Y;Hung JY
通讯作者：
Hung JY
影响因子：
--
作者：
Chen, Yi;Peng, Ya;Huang, Jiefu
通讯作者：
Huang, Jiefu
影响因子：
5.7
作者：
Kondo Y;Shinjo K;Katsushima K
通讯作者：
Katsushima K
下调长链非编码 RNA PART1 抑制膀胱癌增殖并促进细胞凋亡
影响因子：
2.8
作者：
Hu, Xin;Feng, Hefei;Hu, Xiaowen
通讯作者：
Hu, Xiaowen