Schwann Cells in the Ventral Dermis Do Not Derive from Myf5-Expressing Precursors.
Schwann Cells in the Ventral Dermis Do Not Derive from Myf5-Expressing Precursors.
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DOI：
10.1016/j.stemcr.2017.09.010
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发表时间：
2017-11-14
影响因子：
5.9
通讯作者：
Izeta A
中科院分区：
文献类型：
--
作者：
Iribar H;Pérez-López V;Etxaniz U;Gutiérrez-Rivera A;Izeta A
躯干真皮谱系前体的胚胎起源存在一些争议。据报道，由 Myf5 和 Twist2 (Dermo1) 启动子激活追踪的前体细胞（即假定的真皮肌节谱系的细胞）可产生施万细胞。另一方面，大量数据表明真皮雪旺细胞源自神经嵴。这是相关的，因为真皮前体产生神经谱系，并且多谱系分化潜力使它们有资格成为成体干细胞。然而，目前尚不清楚神经谱系是否源自去分化的雪旺细胞而不是中胚层来源的真皮前体细胞。为了澄清这些差异，我们通过两个独立的 Myf5 谱系追踪菌株追踪了 SOX2+ 成体真皮前体细胞。我们证明真皮雪旺细胞不属于 Myf5+ 细胞谱系，这表明之前的追踪数据反映了异常的 cre 重组酶表达，并且真正的 Myf5+ 真皮前体在生理条件下不能转分化为神经谱系。成年 Myf5-creSor 小鼠异常追踪真皮雪旺细胞 (dSC) 去分化的 SOX2+ dSC 是真皮中具有神经活性的前体细胞 这些发现对成人皮肤源性前体细胞的多能性提出了质疑 在本文中，Izeta、Gutiérrez-Rivera 及其同事解决了关于非神经嵴源性成人真皮前体细胞如何能够承担外周神经胶质命运的争议。通过 Myf5+ 细胞谱系追踪（使用两个独立的菌株）、原位定位和分选实验，他们证明异常谱系追踪意外地追踪到了真皮雪旺细胞。因此，成人腹侧真皮的神经活性细胞可能只是去分化的雪旺细胞，而不是常驻干细胞/前体细胞。
The embryonic origin of lineage precursors of the trunk dermis is somewhat controversial. Precursor cells traced by Myf5 and Twist2 (Dermo1) promoter activation (i.e., cells of presumed dermomyotomal lineage) have been reported to generate Schwann cells. On the other hand, abundant data demonstrate that dermal Schwann cells derive from the neural crest. This is relevant because dermal precursors give rise to neural lineages, and multilineage differentiation potential qualifies them as adult stem cells. However, it is currently unclear whether neural lineages arise from dedifferentiated Schwann cells instead of mesodermally derived dermal precursor cells. To clarify these discrepancies, we traced SOX2+ adult dermal precursor cells by two independent Myf5 lineage tracing strains. We demonstrate that dermal Schwann cells do not belong to the Myf5+ cell lineage, indicating that previous tracing data reflected aberrant cre recombinase expression and that bona fide Myf5+ dermal precursors cannot transdifferentiate to neural lineages in physiological conditions. Adult Myf5-creSor mice aberrantly trace dermal Schwann cells (dSCs) Dedifferentiated, SOX2+ dSCs are the neural-competent precursors in the dermis These findings cast doubt on the multipotency of adult skin-derived precursors In this article, Izeta, Gutiérrez-Rivera and colleagues solve a controversy regarding how non-neural crest-derived adult dermal precursors are able to commit to peripheral glial fate. Through Myf5+ cell lineage tracing (with two independent strains), in situ localization, and sorting experiments, they demonstrate that aberrant lineage tracing unexpectedly traces dermal Schwann cells. Thus, instead of resident stem/precursor cells, neural-competent cells of adult ventral dermis may just be dedifferentiated Schwann cells.
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影响因子：
4
作者：
Feisst, Vaughan;Brooks, Anna E. S.;Dunbar, P. Rod
通讯作者：
Dunbar, P. Rod
影响因子：
64.5
作者：
Fujiwara H;Ferreira M;Donati G;Marciano DK;Linton JM;Sato Y;Hartner A;Sekiguchi K;Reichardt LF;Watt FM
通讯作者：
Watt FM
影响因子：
21.3
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
通讯作者：
Miller, FD
影响因子：
5.9
作者：
Etxaniz, Usue;Perez-San Vicente, Adrian;Gago-Lopez, Nuria;Garcia-Dominguez, Mario;Iribar, Haizea;Aduriz, Ariane;Perez-Lopez, Virginia;Burgoa, Izaskun;Irizar, Haritz;Munoz-Culla, Maider;Vallejo-Illarramendi, Ainara;Leis, Olatz;Matheu, Ander;Martin, Angel G.;Otaegui, David;Paz Lopez-Mato, Maria;Gutierrez-Rivera, Araika;MacLellan, Robb;Izeta, Ander
通讯作者：
Izeta, Ander
影响因子：
5.9
作者：
Krause, Matthew P.;Dworski, Shaalee;Feinberg, Konstantin;Jones, Karen;Johnston, Adam P. W.;Paul, Smitha;Paris, Maryline;Peles, Elior;Bagli, Darius;Forrest, Christopher R.;Kaplan, David R.;Miller, Freda D.
通讯作者：
Miller, Freda D.