Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters.
Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters.
复制标题
华盛顿州太平洋牡蛎的嵌套环境条件的嵌套空间和时间建模。
DOI：
10.3389/fmicb.2022.849336
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发表时间：
2022
影响因子：
5.2
通讯作者：
Curriero, Frank C.
中科院分区：
文献类型：
--
作者：
Fries, Brendan;Davis, Benjamin J. K.;Corrigan, Anne E.;DePaola, Angelo;Curriero, Frank C.
关键词：
太平洋西北部（PNW）是美国太平洋牡蛎（Crassostrea gigas）最大的商业捕捞区之一。副溶血性弧菌是一种天然存在于河口沃茨的细菌，在贝类中积累，是引起海产品传播疾病的主要原因。种植者、消费者和公共卫生官员都对该地区弧菌病病例的增加表示担忧。在2005年至2019年5月至10月期间定期采样的华盛顿州太平洋牡蛎（N = 2，836）中，使用最可能数（MPN）-PCR技术估计副溶血性弧菌遗传标记（tlh，tdh和trh）;还在每次采样事件中测量了环境条件。多水平混合效应回归模型被用来评估环境措施和遗传标记之间的关系，以及遗传标记的比例（trh：tlh，tdh：tlh，和tdh：trh），占跨空间和时间的变化。空间和时间的依赖性也占在模型结构。当包括前几周的环境措施时，模型拟合得到改善（气温滞后1周，盐度滞后3周）。在tlh和地表水温度之间发现了正相关，特别是在15和26°C之间，而在trh和地表水温度之间发现了正相关，最高可达26°C。在大多数含盐沃茨（> 27 ppt）中，TLH和TRH与3周滞后盐度呈负相关。组织温度与tdh也有正相关关系，但仅在20 ℃以上tdh：tlh比值显示与tlh类似的反向非线性关系。遗传靶标和环境措施之间的非线性关联表明了副溶血性弧菌复杂的栖息地适应性。与空间和时间变量的其他关联也表明，有影响力的未测量的环境条件，可以进一步解释细菌的变异性。总的来说，这些发现证实了华盛顿州弧菌病先前的生态风险因素，同时也确定了滞后时间效应与副溶血性弧菌致病性标志物之间的新关联。
The Pacific Northwest (PNW) is one of the largest commercial harvesting areas for Pacific oysters (Crassostrea gigas) in the United States. Vibrio parahaemolyticus, a bacterium naturally present in estuarine waters accumulates in shellfish and is a major cause of seafood-borne illness. Growers, consumers, and public-health officials have raised concerns about rising vibriosis cases in the region. Vibrio parahaemolyticus genetic markers (tlh, tdh, and trh) were estimated using an most-probable-number (MPN)-PCR technique in Washington State Pacific oysters regularly sampled between May and October from 2005 to 2019 (N = 2,836); environmental conditions were also measured at each sampling event. Multilevel mixed-effects regression models were used to assess relationships between environmental measures and genetic markers as well as genetic marker ratios (trh:tlh, tdh:tlh, and tdh:trh), accounting for variation across space and time. Spatial and temporal dependence were also accounted for in the model structure. Model fit improved when including environmental measures from previous weeks (1-week lag for air temperature, 3-week lag for salinity). Positive associations were found between tlh and surface water temp, specifically between 15 and 26°C, and between trh and surface water temperature up to 26°C. tlh and trh were negatively associated with 3-week lagged salinity in the most saline waters (> 27 ppt). There was also a positive relationship between tissue temperature and tdh, but only above 20°C. The tdh:tlh ratio displayed analogous inverted non-linear relationships as tlh. The non-linear associations found between the genetic targets and environmental measures demonstrate the complex habitat suitability of V. parahaemolyticus. Additional associations with both spatial and temporal variables also suggest there are influential unmeasured environmental conditions that could further explain bacterium variability. Overall, these findings confirm previous ecological risk factors for vibriosis in Washington State, while also identifying new associations between lagged temporal effects and pathogenic markers of V. parahaemolyticus.
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影响因子：
6.1
作者：
GODFREY, LG
通讯作者：
GODFREY, LG
影响因子：
1
作者：
Ben-Horin, Tal;Audemard, Corinne;Bushek, David
通讯作者：
Bushek, David
影响因子：
5.2
作者：
Flynn, Aspen;Davis, Benjamin J. K.;Curriero, Frank C.
通讯作者：
Curriero, Frank C.
影响因子：
4.4
作者：
Jones, J. L.;Kinsey, T. P.;Bowers, J. C.
通讯作者：
Bowers, J. C.
影响因子：
1.9
作者：
BREUSCH, TS
通讯作者：
BREUSCH, TS