Immunization with matrix-, nucleoprotein and neuraminidase protects against H3N2 influenza challenge in pH1N1 pre-exposed pigs.
Immunization with matrix-, nucleoprotein and neuraminidase protects against H3N2 influenza challenge in pH1N1 pre-exposed pigs.
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DOI：
10.1038/s41541-023-00620-2
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发表时间：
2023-02-15
期刊：
影响因子：
9.2
通讯作者：
中科院分区：
文献类型：
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作者：
迫切需要提供更广泛保护的流感疫苗，这可能减少对人群年度免疫的需要。我们研究了用黑猩猩腺病毒（ChAdOx2）和修饰的安卡拉牛痘（MVA）载体疫苗在H1N1pdm09预暴露猪中进行异源初免加强免疫的效力，所述载体疫苗表达保守的流感病毒核蛋白（NP）、基质蛋白1（M1）和神经氨酸酶（NA）。我们比较了鼻内、气雾剂和肌肉注射疫苗对H3N2流感攻击的效力。H3 N2攻击后，气溶胶初免加强免疫诱导了强烈的局部肺T细胞和抗体反应，并消除了病毒脱落和肺部病理。相比之下，肌肉内免疫诱导了强有力的全身反应和弱的局部肺反应，但也消除了肺病理学并减少了病毒脱落。这些结果为在高度相关的大型动物模型中开发广泛保护性流感疫苗提供了有价值的见解，并将为未来的疫苗和临床试验设计提供信息。
There is an urgent need for influenza vaccines providing broader protection that may decrease the need for annual immunization of the human population. We investigated the efficacy of heterologous prime boost immunization with chimpanzee adenovirus (ChAdOx2) and modified vaccinia Ankara (MVA) vectored vaccines, expressing conserved influenza virus nucleoprotein (NP), matrix protein 1 (M1) and neuraminidase (NA) in H1N1pdm09 pre-exposed pigs. We compared the efficacy of intra-nasal, aerosol and intra-muscular vaccine delivery against H3N2 influenza challenge. Aerosol prime boost immunization induced strong local lung T cell and antibody responses and abrogated viral shedding and lung pathology following H3N2 challenge. In contrast, intramuscular immunization induced powerful systemic responses and weak local lung responses but also abolished lung pathology and reduced viral shedding. These results provide valuable insights into the development of a broadly protective influenza vaccine in a highly relevant large animal model and will inform future vaccine and clinical trial design.
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DOI：
10.1093/cid/cis327
发表时间：
2012-07
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Lillie PJ;Berthoud TK;Powell TJ;Lambe T;Mullarkey C;Spencer AJ;Hamill M;Peng Y;Blais ME;Duncan CJ;Sheehy SH;Havelock T;Faust SN;Williams RL;Gilbert A;Oxford J;Dong T;Hill AV;Gilbert SC
通讯作者：
Gilbert SC
影响因子：
4.4
作者：
Holzer, Barbara;Morgan, Sophie B.;Tchilian, Elma
通讯作者：
Tchilian, Elma
DOI：
10.1038/mt.2013.284
发表时间：
2014-03
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
通讯作者：
--
DOI：
10.4049/jimmunol.2001086
发表时间：
2021-02-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Martini V;Paudyal B;Chrun T;McNee A;Edmans M;Atangana Maze E;Clark B;Nunez A;Dolton G;Sewell A;Beverley P;MacLoughlin R;Townsend A;Tchilian E
通讯作者：
Tchilian E
DOI：
10.1164/rccm.201411-1988oc
发表时间：
2015-06-15
影响因子：
24.7
作者：
Hayward, Andrew C.;Wang, Lili;McMichael, Andrew J.
通讯作者：
McMichael, Andrew J.