Low-level laser (light) therapy increases mitochondrial membrane potential and ATP synthesis in C2C12 myotubes with a peak response at 3-6 h.
Low-level laser (light) therapy increases mitochondrial membrane potential and ATP synthesis in C2C12 myotubes with a peak response at 3-6 h.
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低水平激光（光）治疗增加了C2C12肌管中的线粒体膜电位和ATP合成，在3-6 h时峰值响应。
DOI：
10.1111/php.12397
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发表时间：
2015-03
影响因子：
3.3
通讯作者：
Hamblin MR
中科院分区：
文献类型：
--
作者：
Ferraresi C;Kaippert B;Avci P;Huang YY;de Sousa MV;Bagnato VS;Parizotto NA;Hamblin MR
低水平激光（光）疗法已在运动前用于提高实验动物和人类的肌肉性能。然而，关于在运动前应用光的最佳时间存在不确定性。其作用机制被认为是刺激肌肉中的线粒体呼吸，并增加运动所需的三磷酸腺苷（ATP）。本研究的目的是调查的线粒体膜电位（MMP）和ATP的增加，从C2 C12小鼠肌细胞形成的肌管，并暴露于发光二极管治疗（LEDT）的时间过程。LEDT采用具有20个红色（630 ± 10 nm，25 mW）和20个近红外（850 ± 10 nm，50 mW）的LED簇，提供28 mW/cm 2持续90秒（2.5 J/cm 2），在LEDT后5分钟、3小时、6小时和24小时进行分析。LEDT-6 h的MMP最高，其次是LEDT-3 h、LEDT-24 h、LEDT-5 min和对照，差异显著。ATP含量顺序为6 h> 3 h> 24 h> 5 min>对照组，差异有显著性。MMP与ATP之间存在良好的相关性（r=0.89）。这些数据表明LEDT刺激肌细胞的最佳时间窗为3-6 h。
Low level laser (light) therapy has been used before exercise to increase muscle performance in both experimental animals and in humans. However uncertainty exists concerning the optimum time to apply the light before exercise. The mechanism of action is thought to be stimulation of mitochondrial respiration in muscles, and to increase adenosine triphosphate (ATP) needed to perform exercise. The goal of this study was to investigate the time course of the increases in mitochondrial membrane potential (MMP) and ATP in myotubes formed from C2C12 mouse muscle cells and exposed to light-emitting diode therapy (LEDT). LEDT employed a cluster of LEDs with 20 red (630 ± 10 nm, 25 mW) and 20 near-infrared (850 ± 10 nm, 50 mW) delivering 28 mW/cm2 for 90 sec (2.5 J/cm2) with analysis at 5 min, 3 h, 6 h and 24 h post-LEDT. LEDT-6h had the highest MMP, followed by LEDT-3h, LEDT-24h, LEDT-5min and Control with significant differences. The same order (6h>3h>24h>5min>Control) was found for ATP with significant differences. A good correlation was found (r=0.89) between MMP and ATP. These data suggest an optimum time window of 3-6 h for LEDT stimulate muscle cells.
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影响因子：
7
作者：
Tannu, NS;Rao, VK;Raghow, R
通讯作者：
Raghow, R
DOI：
10.1016/1011-1344(94)07078-3
发表时间：
1995-03-01
影响因子：
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作者：
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通讯作者：
Calza, Laura