The transcriptome difference between vulnerable and stable carotid atherosclerotic plaque
The transcriptome difference between vulnerable and stable carotid atherosclerotic plaque
复制标题
易损性和稳定性颈动脉粥样硬化斑块转录组差异
DOI：
--
复制
发表时间：
2018
影响因子：
0.1
通讯作者：
Chengxiong Gu
中科院分区：
文献类型：
--
作者：
Tongxun Li;Xiaoping Zhang;Jinqian Zhang;Rui Liu;Chengxiong Gu
近年来，动脉粥样硬化斑块的不稳定性是基础和临床研究的热点。准确识别易损斑块，并在急性心血管事件发生前进行有效干预是非常必要的。本研究收集了动脉粥样硬化斑块和易损斑块的稳定性数据，采用新一代RNA-seq技术进行分析。我们使用Illumina深度测序技术，通过下一代测序（NGS）对易损和稳定的颈动脉粥样硬化斑块进行了转录组序列分析。我们分别从三个脆弱的颈动脉粥样硬化斑块中获得5，473，799、3，766，914、7，713，565个合格的Illumina读数，或从稳定的颈动脉粥样硬化斑块中获得5，598，305、7，249，629和5，623，320个合格的Illumina读数。比较转录组差异分析显示，易损斑块与稳定斑块之间有781条基因表达上调，318条基因表达上调，363条基因表达下调。火山图用于进一步确定两组之间的差异基因表达。进行聚类分析，并计算相关系数进行分析。我们的工作证明了脆弱和稳定的颈动脉粥样硬化斑块之间的差异转录组。大量的基因将成为揭示与动脉粥样硬化斑块的病理生理和发病机制相关的表达调控分子机制的一个有前途的资源，甚至它们在治疗领域的意义
In recent years, the instability of atherosclerotic plaques is a hotspot of basic and clinical research. Moreover,.it is imperative necessary to accurately identify the vulnerable plaques, and give effectively intervention before.acute cardiovascular events. In this work, the stability of atherosclerotic plaques and vulnerable plaques were collected.and performed with next-generation of RNA-seq, then data were analyzed. We performed a transcriptome.sequence analysis of the vulnerable and stable carotid atherosclerotic plaque by next generation sequencing (NGS).using the Illumina Deep Sequencing technology. We obtained 5,473,799, 3,766,914, 7,713,565 qualified Illumina.reads from three vulnerable carotid atherosclerotic plaque, or 5,598,305, 7,249,629, and 5,623,320 qualified.Illumina reads from stable carotid atherosclerotic plaque, respectively. Comparative trancriptome analysis differentially.revealed 781 genes between vulnerable and stable carotid atherosclerotic plaque, 318 expression of genes.was up-regulated and 363 expressions of genes were down-regulated. The volcano plot was used to further identify.differential genes expression between the two groups. Cluster analysis was performed, and spearman correlation.coefficient was calculated and analyzed. Our work demonstrated differential transcriptomes between vulnerable.and stable carotid atherosclerotic plaque. Numbers of genes will serve as a promising resource for revealing the.regulatory molecular mechanisms of expression associated with the pathophysiology and pathogenesis of vulnerable.atherosclerosis plaque, even their implications in the field of therapy
登录
查看更多内容
DOI：
10.1016/b978-0-12-809633-8.20106-4
发表时间：
2020-02
期刊：
--
影响因子：
--
作者：
A. Prjibelski;Anton I. Korobeynikov;Alla L. Lapidus
通讯作者：
A. Prjibelski;Anton I. Korobeynikov;Alla L. Lapidus
影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
46.9
作者：
Patro, Rob;Mount, Stephen M.;Kingsford, Carl
通讯作者：
Kingsford, Carl
影响因子：
4.4
作者：
Zaid M;Fujiyoshi A;Kadota A;Abbott RD;Miura K
通讯作者：
Miura K