Repetitive reduction lead to significant elevated IL-6 and decreased IL-10 levels in femoral osteotomies: A quantitative analysis of a robot-assisted reduction process in a rat model.
Repetitive reduction lead to significant elevated IL-6 and decreased IL-10 levels in femoral osteotomies: A quantitative analysis of a robot-assisted reduction process in a rat model.
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股骨截骨术中重复复位导致 IL-6 显着升高和 IL-10 水平降低：大鼠模型中机器人辅助复位过程的定量分析
DOI：
10.1016/j.injury.2016.05.017
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发表时间：
2016
期刊：
影响因子：
2.5
通讯作者：
C. Neunaber
中科院分区：
文献类型：
--
作者：
P. Haas;H. Wypior;R. Westphal;M. Petri;F. Hildebrand;T. Goesling;C. Krettek;C. Neunaber
机器人辅助骨折复位领域已经由几个研究小组开发了十多年，主要目标是提高骨折复位的准确性。然而，不同复位路径对患者生理的影响尚不完全清楚。我们研究的目的是通过测量anin vivorat model.Materials和methods 36只雄性CD©大鼠分为三组，每组在左股骨上使用外固定器和截骨术，以比较机器人辅助直接复位路径与人工延长复位路径的影响。七天后，机器人被连接，一组在一次尝试中被减少，而另一组经历了机器人的10次尝试。第三组为对照组，不复位。在还原过程之前以及还原过程之后6、24和48 h采集血样。IL-1、IL-6、IL-10、IL-17和MCP-1浓度通过ELISA或细胞计数珠测定法进行分析。在复位过程后48小时收集截骨区域的肌肉活检用于组织学分析。结果细胞因子分析显示，与对照组相比，减少10次尝试后6 h，促炎细胞因子IL-6显著升高。与单次尝试复位组相比，10次尝试复位组术后6 h的IL-6水平进一步显著升高。在抗炎方面，与单次尝试减少组和对照组相比，在减少后6小时，十次尝试减少组中的IL-10显示出显著降低。肌肉活检显示，在两个减少组的病理变化显着增加，并增加了10尝试减少组相比，单尝试减少和control group.ConclusionA直接和温和的减少程序可行的机器人的帮助下，在细胞因子的反应和组织变化方面的长期减少。
IntroductionThe field of robot-assisted fracture reduction has been developed by several research groups over more than one decade by now, with the main goals of increasing the fracture reduction accuracy. However, the influence of different reduction paths to patients’ physiology is not fully known yet. The aim of our study was to compare the impacts of a robot-assisted direct reduction path versus an artificially prolonged reduction path by measuring the cytokine responses in anin vivorat model.Materials and methodsThirty-six male CD©rats were assigned into three groups with an external fixator and osteotomy on the left femur. Seven days later, the robot was attached and one group was reduced in a single attempt, while the other group underwent 10 attempts by the robot. The third group was the control group without reduction. Before, and as well as 6, 24 and 48 h after the reduction process blood samples were collected. IL-1, IL-6, IL-10, IL-17, and MCP-1 concentrations where analysed via ELISA or cytometric bead assay. Muscle biopsies in the osteotomy area were collected 48 h after the reduction process for histological analyses. Statistical significance was set at p ≤ 0.05.ResultsAnalysis of the cytokines showed that the pro-inflammatory cytokine IL-6 of the Ten-Attempts reduction group significantly increased 6 h after reduction compared to the control group. IL-6 further showed markedly elevated levels 6 h after surgery in the Ten-Attempts reduction group compared to the Single-Attempt reduction group. On the anti-inflammatory side, IL-10 showed a significant decrease in the Ten-Attempts reduction group 6 h after reduction compared to the Single-Attempt reduction and control group. Muscle biopsies showed a significant increase of pathological changes in both reduction groups and an increase in the severity of bleedings of the Ten-Attempts reduction group compared to the Single-Attempt reduction and control group.ConclusionA direct and gentle reduction procedure as feasible by the aid of a robot is preferable over a prolonged reduction in terms of cytokine response and tissue changes.
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影响因子：
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作者：
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影响因子：
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作者：
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发表时间：
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影响因子：
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作者：
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DOI：
--
发表时间：
2005
期刊：
Der Unfallchirurg
影响因子：
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作者：
P. Weninger;H. Trimmel;T. Nau;S. Aldrian;F. König;V. Vécsei
通讯作者：
V. Vécsei