Interplay between Endometriosis and Pregnancy in a Mouse Model.
Interplay between Endometriosis and Pregnancy in a Mouse Model.
复制标题
DOI：
10.1371/journal.pone.0124900
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Barañao RI
中科院分区：
文献类型：
--
作者：
Bilotas MA;Olivares CN;Ricci AG;Baston JI;Bengochea TS;Meresman GF;Barañao RI
目的：探讨子宫内膜异位症对小鼠生育力及腹腔液中IL-2和干扰素-γ水平的影响，以及妊娠对子宫内膜异位症病变生长、细胞凋亡和细胞增殖的影响。两个月龄的C57BL/6雌性小鼠接受了诱发子宫内膜异位症的手术或假手术。手术后4周，小鼠交配，并在怀孕第18天处死。记录着床部位数、胎次和胎重。对子宫内膜异位病变进行计数、测量、切除和固定。采用原位末端标记法和增殖细胞核抗原免疫组织化学方法分别检测皮损中细胞的凋亡和增殖情况。用双抗体夹心法测定腹腔液中IL-2和干扰素-γ的水平。子宫内膜异位症小鼠的妊娠率(即怀孕小鼠/N)下降。但在吸收率、窝产仔数和仔猪体重方面，各组间差异不显著。干扰素-γ在子宫内膜异位症小鼠中增强，与妊娠结局无关。此外，与妊娠假手术组相比，妊娠子宫内膜异位症小鼠的干扰素-γ增加。假手术组未孕小鼠干扰素-γ较妊娠组升高，子宫内膜异位症组未孕小鼠IL-2升高。妊娠期小鼠子宫内膜异位灶体积增大，间质细胞凋亡率增加，上皮细胞增殖减少。此外，在相同的皮损中，白细胞浸润、坏死和蜕膜化增加。在这种子宫内膜异位症的小鼠模型中，妊娠率降低。子宫内膜异位症小鼠腹腔液中IL-2水平升高，提示该细胞因子在与该病相关的不孕症中起作用。妊娠小鼠子宫内膜异位病变的大小增加；然而，怀孕通过减少细胞增殖，通过增加细胞凋亡、蜕膜化和坏死而对病变产生有益的影响。
To evaluate the effect of endometriosis on fertility and the levels of the IL-2 and IFN-γ in the peritoneal fluid in a mouse model; to evaluate the effect of pregnancy on endometriotic lesion growth, apoptosis and cell proliferation. Two month old C57BL/6 female mice underwent either a surgical procedure to induce endometriosis or a sham surgery. Four weeks after surgery mice were mated and sacrificed at day 18 of pregnancy. Number of implantation sites, fetuses and fetal weight were recorded. Endometriotic lesions were counted, measured, excised and fixed. Apoptosis and cell proliferation were evaluated in lesions by TUNEL and immunohistochemistry for PCNA respectively. Levels of IL-2 and IFN-γ were assessed by ELISA in the peritoneal fluid. Pregnancy rate (i.e. pregnant mice/N) decreased in mice with endometriosis. However there were no significant differences in resorption rate, litter size and pup weight between groups. IFN-γ augmented in endometriosis mice independently of pregnancy outcome. Additionally IFN-γ increased in pregnant endometriosis mice compared to pregnant sham animals. While IFN-γ increased in non pregnant versus pregnant mice in the sham group, IL-2 was increased in non pregnant mice in the endometriosis group. The size of endometriotic lesions increased in pregnant mice while apoptosis increased in the stroma and cell proliferation decreased in the epithelium of these lesions. Additionally, leukocyte infiltration, necrosis and decidualization were increased in the same lesions. Pregnancy rate is reduced in this mouse model of endometriosis. Levels of IL-2 are increased in the peritoneal fluid of mice with endometriosis suggesting a role of this cytokine in infertility related to this disease. The size of endometriotic lesions is increased in pregnant mice; however pregnancy has a beneficial effect on lesions by decreasing cell proliferation and by increasing apoptosis, decidualization and necrosis.
登录
查看更多内容
影响因子：
3.2
作者：
Macer ML;Taylor HS
通讯作者：
Taylor HS
影响因子：
6.1
作者：
MAHMOOD, TA;TEMPLETON, A
通讯作者：
TEMPLETON, A
在细胞周期中存在两个细胞周期蛋白/增殖细胞核抗原的种群：与DNA复制位点的关联。
影响因子：
7.8
作者：
Bravo, R;Macdonald-Bravo, H
通讯作者：
Macdonald-Bravo, H
影响因子：
9.8
作者：
Katorza, E.;Soriano, D.;Goldenberg, M.
通讯作者：
Goldenberg, M.
影响因子：
5.8
作者：
Katsuki, Y;Takano, Y;Nozawa, S
通讯作者：
Nozawa, S