Dimensions of a Living Cochlear Hair Bundle.
Dimensions of a Living Cochlear Hair Bundle.
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DOI：
10.3389/fcell.2021.742529
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发表时间：
2021
影响因子：
5.5
通讯作者：
Grillet N
中科院分区：
文献类型：
--
作者：
Miller KK;Atkinson P;Mendoza KR;Ó Maoiléidigh D;Grillet N
毛束是毛细胞的机械感觉细胞器，其检测由声音、头部运动和流体流动引起的机械刺激。每个毛束都是一个叫做静纤毛的细胞突起的集合体，它们的高度不同，形成一个楼梯。在不同的物种、感觉器官、器官内的位置、毛细胞类型，甚至在单个毛束内，静纤毛具有不同的高度、宽度和间隔。立体纤毛组件的尺寸决定了发束如何响应刺激。这些发束特性以前只在有限的程度上被测量过。特别是，哺乳动物的数据要么是不完整的，缺乏对年龄或器官内位置的控制，要么是由于固定或脱水而产生的假象。在这里，我们提供了一套完整的测量出生后（P）11天C57 BL/6 J小鼠顶端内毛细胞（IHC）从活组织，组织轻度固定的荧光成像，或组织强烈固定和脱水的扫描电子显微镜（SEM）。我们发现，发束轻度固定的荧光具有相同的尺寸作为活的发束，而SEM制备的发束收缩均匀的立纤毛的高度，宽度和分离。通过确定收缩因子，我们从SEM估算了太小而无法光学观察的活尺寸。因此，我们创建了第一个完整的活IHC毛束蓝图。我们表明，SEM准备的测量强烈影响束的机械性能的计算-高估静纤毛偏转刚度和低估静纤毛之间的流体耦合。我们描述的测量方法，数据和结果说明了理解毛束机械转导所需的高水平的准确性和精度。
The hair bundle is the mechanosensory organelle of hair cells that detects mechanical stimuli caused by sounds, head motions, and fluid flows. Each hair bundle is an assembly of cellular-protrusions called stereocilia, which differ in height to form a staircase. Stereocilia have different heights, widths, and separations in different species, sensory organs, positions within an organ, hair-cell types, and even within a single hair bundle. The dimensions of the stereociliary assembly dictate how the hair bundle responds to stimuli. These hair-bundle properties have been measured previously only to a limited degree. In particular, mammalian data are either incomplete, lack control for age or position within an organ, or have artifacts owing to fixation or dehydration. Here, we provide a complete set of measurements for postnatal day (P) 11 C57BL/6J mouse apical inner hair cells (IHCs) obtained from living tissue, tissue mildly-fixed for fluorescent imaging, or tissue strongly fixed and dehydrated for scanning electronic microscopy (SEM). We found that hair bundles mildly-fixed for fluorescence had the same dimensions as living hair bundles, whereas SEM-prepared hair bundles shrank uniformly in stereociliary heights, widths, and separations. By determining the shrinkage factors, we imputed live dimensions from SEM that were too small to observe optically. Accordingly, we created the first complete blueprint of a living IHC hair bundle. We show that SEM-prepared measurements strongly affect calculations of a bundle’s mechanical properties – overestimating stereociliary deflection stiffness and underestimating the fluid coupling between stereocilia. The methods of measurement, the data, and the consequences we describe illustrate the high levels of accuracy and precision required to understand hair-bundle mechanotransduction.
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影响因子：
4.6
作者：
Asai Y;Pan B;Nist-Lund C;Galvin A;Lukashkin AN;Lukashkina VA;Chen T;Zhou W;Zhu H;Russell IJ;Holt JR;Géléoc GSG
通讯作者：
Géléoc GSG
影响因子：
3.7
作者：
Smith ST;Chadwick RS
通讯作者：
Chadwick RS
影响因子：
16.6
作者：
Corns LF;Johnson SL;Roberts T;Ranatunga KM;Hendry A;Ceriani F;Safieddine S;Steel KP;Forge A;Petit C;Furness DN;Kros CJ;Marcotti W
通讯作者：
Marcotti W
DOI：
10.1073/pnas.1902163116
发表时间：
2019-05-28
影响因子：
11.1
作者：
Bartsch, Tobias F.;Hengel, Felicitas E.;Hudspeth, A. J.
通讯作者：
Hudspeth, A. J.
影响因子：
3.4
作者：
Beurg, Maryline;Nam, Jong-Hoon;Fettiplace, Robert
通讯作者：
Fettiplace, Robert