A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development.
A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development.
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DOI：
10.1007/s00122-011-1578-8
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发表时间：
2011-07
影响因子：
5.4
通讯作者：
Donini, Paolo
中科院分区：
文献类型：
--
作者：
Bindler, Gregor;Plieske, Joerg;Bakaher, Nicolas;Gunduz, Irfan;Ivanov, Nikolai;Van der Hoeven, Rutger;Ganal, Martin;Donini, Paolo
烟草（Nicotiana tabacum L.）是茄科（Solanaceae）大家族中的一个物种，并且作为农艺作物和植物生物技术中的模式系统是重要的。尽管其重要性，只有有限的分子标记资源可用于基因组分析，遗传作图和育种。我们在这里报告的5,119个新的和功能性微卫星标记的开发和表征和四倍体烟草基因组的高分辨率遗传图谱的生成。遗传图谱的生成使用来自希克斯阔叶×红俄罗斯品种间杂交的F2作图群体，并将来自这个新集合的多态性标记与来自先前用于产生较低密度图谱的较小集合的多态性标记合并。这里描述的遗传图谱包含2，317个微卫星标记和2，363个基因座，导致绘制的微卫星标记之间的平均距离小于200万个碱基对或1.5 cM。有了这种新的和扩展的标记资源，足够数量的标记现在可用于从烟草育种到比较基因组分析的多种应用。烟草的遗传图谱在标记密度和分辨率方面与茄科的最佳特征基因组：番茄和马铃薯相当。本文的在线版本（doi：10.1007/s 00122 -011-1578-8）包含补充材料，可供授权用户使用。
Tobacco (Nicotiana tabacum L.) is a species in the large family of the Solanaceae and is important as an agronomic crop and as a model system in plant biotechnology. Despite its importance, only limited molecular marker resources are available that can be used for genome analysis, genetic mapping and breeding. We report here on the development and characterization of 5,119 new and functional microsatellite markers and on the generation of a high-resolution genetic map for the tetraploid tobacco genome. The genetic map was generated using an F2 mapping population derived from the intervarietal cross of Hicks Broadleaf × Red Russian and merges the polymorphic markers from this new set with those from a smaller set previously used to produce a lower density map. The genetic map described here contains 2,317 microsatellite markers and 2,363 loci, resulting in an average distance between mapped microsatellite markers which is less than 2 million base pairs or 1.5 cM. With this new and expanded marker resource, a sufficient number of markers are now available for multiple applications ranging from tobacco breeding to comparative genome analysis. The genetic map of tobacco is now comparable in marker density and resolution with the best characterized genomes of the Solanaceae: tomato and potato. The online version of this article (doi:10.1007/s00122-011-1578-8) contains supplementary material, which is available to authorized users.
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