Effects of active oxygen species and methyl jasmonate on expression of the gene for a wound-inducible 1-aminocyclopropane-1-carboxylate synthase in winter squash (Cucurbita maxima)
Effects of active oxygen species and methyl jasmonate on expression of the gene for a wound-inducible 1-aminocyclopropane-1-carboxylate synthase in winter squash (Cucurbita maxima)
复制标题
活性氧和茉莉酸甲酯对冬瓜（Cucurbita maxima）伤口诱导型 1-氨基环丙烷-1-羧酸合酶基因表达的影响
作者：
T. Watanabe;S. Sakai
抽象的。机械损伤诱导西葫芦中果皮组织产生乙烯。从南瓜（Cucurbita maxima Duch.）通过逆转录酶-聚合酶链反应，使用引物，对应于在1-氨基环丙烷-1-羧酸（ACC）酶中发现的两个保守氨基酸序列的水果。WSACS 2的cDNA序列与N. Nakajima等人1990，Plant Cell Physiol 31：1021-1029，仅具有一个核苷酸差异。通过北方印迹分析检测WSACS 2转录物在组织圆片中的表达。WSACS 2 mRNA在受伤组织盘中的积累被二苯碘鎓处理抑制，二苯碘鎓被认为是产生超氧化物的NAD（P）H氧化酶的抑制剂。加入黄嘌呤和黄嘌呤氧化酶，形成一个超氧化物生成系统，强烈诱导WSACS 2转录物的积累在30分钟内和转录物的水平下降之后。我们通过使用化学发光试剂测量了组织圆盘中产生的活性氧物质的水平，并发现化学发光信号在受伤后几秒钟内变得明显。WSACS 2转录物的合成受到脂氧合酶抑制剂的轻微抑制，脂氧合酶参与茉莉酸的合成。茉莉酸甲酯刺激WSACS 2转录物在组织盘中的合成。
Abstract. The production of ethylene by mesocarp tissue of winter squash is induced by mechanical wounding. We isolated a cDNA fragment (WSACS2) from the wounded mesocarp tissue of winter-squash (Cucurbita maxima Duch.) fruit by the reverse transcriptase-polymerase chain reaction using primers that corresponded to two conserved amino acid sequences found in 1-aminocyclopropane-1-carboxylate (ACC) synthases. The sequence of WSACS2 cDNA was almost identical to that of the cDNA for a wound-inducible ACC synthase (CM-ACS1) reported by N. Nakajima et␣al. 1990, Plant Cell Physiol 31: 1021–1029, with only one nucleotide difference. The expression of the WSACS2 transcript in tissue disks was examined by Northern blotting analysis. The accumulation of WSACS2 mRNA in the wounded tissue disks was inhibited by treatment with diphenyleneiodonium, which is known as an inhibitor of superoxide-generating NAD(P)H oxidases. The addition of xanthine and xanthine oxidase, which form a superoxide-generating system, strongly induced the accumulation of the WSACS2 transcript within 30 min and the level of the transcript fell thereafter. We measured levels of active oxygen species that were generated in the tissue disks by using chemiluminescence reagents and found that the chemiluminescence signal became apparent within a few seconds after wounding. The synthesis of the WSACS2 transcript was slightly inhibited by inhibitors of lipoxygenase, which is involved in the synthesis of jasmonic acid. Methyl jasmonate stimulated the synthesis of the WSACS2 transcript in the tissue disks.
DOI：
10.1073/pnas.89.22.11046
发表时间：
1992-11-15
影响因子：
11.1
作者：
LIANG, XW;ABEL, S;THEOLOGIS, A
通讯作者：
THEOLOGIS, A
DOI：
10.1073/pnas.86.17.6621
发表时间：
1989-09-01
影响因子：
11.1
作者：
SATO, T;THEOLOGIS, A
通讯作者：
THEOLOGIS, A