OGG1 contributes to hepatocellular carcinoma by promoting cell cycle-related protein expression and enhancing DNA oxidative damage repair in tumor cells.
OGG1 contributes to hepatocellular carcinoma by promoting cell cycle-related protein expression and enhancing DNA oxidative damage repair in tumor cells.
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OGG1通过促进细胞周期相关蛋白表达并增强肿瘤细胞中DNA氧化损伤修复来促进肝细胞癌
DOI：
10.1002/jcla.24561
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发表时间：
2022-07
影响因子：
2.7
通讯作者：
Chen, Jie
中科院分区：
文献类型：
--
作者：
Zhang, He;Jiang, Peng-jun;Lv, Meng-yuan;Zhao, Yan-hua;Cui, Ju;Chen, Jie
本研究旨在通过生物信息学技术分析8-氧代鸟嘌呤DNA糖基化酶（OGG 1）在肝细胞癌（HCC）患者中的表达及其对预后的影响，并通过数据挖掘确定其可能的致癌机制。通过TCGA和GEO数据库检索并分析OGG 1在健康人和HCC患者中表达的差异，通过生存分析判断OGG 1对预后的影响。同时，通过GO分析、KEGG分析、免疫浸润分析、蛋白-蛋白相互作用网络、启动子甲基化分析等，探讨OGG 1在肝癌发生发展中的可能分子机制。采用定量聚合酶链反应（qPCR）检测36对肝癌组织及癌旁组织中的基因表达。OGG 1在HCC患者中的表达高于健康人，OGG 1的过表达可能通过增加细胞周期相关蛋白的活性来刺激细胞增殖。OGG 1的表达与肝癌的发生、发展密切相关。OGG 1有望成为评估HCC预后的新生物标志物和HCC治疗的新靶点。图1 HCC中OGG 1的表达，（A）核浆中OGG 1的表达，（B，C）健康肝组织中OGG 1的表达，（D，E，F）HCC患者的肝组织中的OGG 1表达（G）基于样品类型的HCC中的OGG 1表达（H）淋巴结转移状态（I）TP 53突变状态（J）年龄（K）性别（L）种族（M）分期（N）等级（O）组织学亚型
This study aimed to analyze the expression of 8‐oxoguanine DNA glycosylase (OGG1) in patients with hepatocellular carcinoma (HCC) and its effect on prognosis by bioinformatics techniques and to determine its possible carcinogenic mechanism through data mining. The difference in OGG1 expression between healthy people and HCC patients was searched and analyzed by TCGA and GEO databases, and the effect of OGG1 on prognosis was judged by survival analysis. Meanwhile, the possible molecular mechanism of OGG1 in the tumorigenesis and development of HCC was explored by GO analysis, KEGG analysis, immune infiltration analysis, protein–protein interaction network, promoter methylation analysis, and so forth. Quantitative polymerase chain reaction (qPCR) was used to examine the gene expression in 36 pairs of HCC tissues and adjacent tissues. The expression of OGG1 in HCC patients was higher than that in healthy people, and the overexpression of OGG1 might stimulate cell proliferation by increasing the activity of cell cycle‐related proteins. The alteration of OGG1 was significantly correlated with the tumorigenesis and development of HCC. OGG1 is expected to be a new biomarker for evaluating the prognosis of HCC and a new target for the treatment of HCC. Figure 1 Expression of OGG1 in HCC, (A) expression of OGG1 in nucleoplasma, (B, C) OGG1 expression in healthy liver tissues, (D, E, F) OGG1 expression in liver tissues of HCC patients (G) expression of OGG1 in HCC based on sample type (H) nodal metastasis status (I) TP53 mutation status (J) age (K) gender (L) race (M) stage (N) grade (O) histological subtype.
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影响因子：
2.9
作者：
Guan Q;Chen Z;Chen Q;Zhi X
通讯作者：
Zhi X
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2
作者：
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5.6
作者：
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3.7
作者：
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通讯作者：
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作者：
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