Elevated circulating miR-150 and miR-342-3p in patients with irritable bowel syndrome.
Elevated circulating miR-150 and miR-342-3p in patients with irritable bowel syndrome.
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DOI：
10.1016/j.yexmp.2014.04.009
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发表时间：
2014-06
影响因子：
3.6
通讯作者：
Henderson, Wendy A.
中科院分区：
文献类型：
--
作者：
Fourie, Nicolaas H.;Peace, Ralph Michael;Abey, Sarah K.;Sherwin, LeeAnne B.;Rahim-Williams, Bridgett;Smyser, Paul A.;Wiley, John W.;Henderson, Wendy A.
微小RNA（miRNAs）是小的非编码RNA，其调节基因表达，因此作为诊断标志物以及作为病因学和干预靶点的线索而令人感兴趣。这项初步研究检查了循环miRNA是否在IBS患者中差异表达。miRNA微阵列（Nanostring）在43名参与者的全血上运行。与健康对照相比，发现IBS患者中hsa-miR-150和hsa-miR-342- 3 p显著升高（FDR调整的p ≤ 0.05，≥1.6倍变化）。这些miRNAs都没有显示出与种族或性别的任何关系。hsa-miR-150与炎症性肠病和疼痛相关，并与蛋白激酶（AKT 2）相互作用，通过该蛋白激酶可影响炎症途径。预测hsa-miR-342- 3 p与参与疼痛信号传导、结肠运动和平滑肌功能的mRNA相互作用。这项初步研究报告了在全血中检测到的两种miRNAs与IBS的相关性。这些miRNAs与疼痛和炎症通路相关，这两者都被认为在IBS中失调。需要更大的样本量来确认它们作为生物标志物的重要性和潜力。
MicroRNAs (miRNAs) are small non-coding RNAs, which regulate gene expression and are thus of interest as diagnostic markers, and as clues to etiology and targets of intervention. This pilot study examined whether circulating miRNAs are differentially expressed in patients with IBS. miRNA microarrays (Nanostring) were run on the whole blood of 43 participants. hsa-miR-150 and hsa-miR-342-3p were found to be significantly elevated (FDR adjusted p ≤ 0.05, ≥1.6 fold change) in IBS patients compared to healthy controls. Neither of these miRNAs showed any relationship to race or sex. hsa-miR-150 is associated with inflammatory bowel disorders and pain, and interacts with a protein kinase (AKT2) through which it may affect inflammatory pathways. hsa-miR-342-3p is predicted to interact with mRNAs involved in pain signaling, colonic motility, and smooth muscle function. This preliminary study reports the association of two miRNAs, detected in whole blood, with IBS. These miRNAs link to pain and inflammatory pathways both of which are thought to be dysregulated in IBS. Larger samples sizes are needed to confirm their importance and potential as biomarkers.
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影响因子：
3.2
作者：
Miwa, J;Echizen, H;Umeda, N
通讯作者：
Umeda, N
DOI：
10.4291/wjgp.v3.i6.102
发表时间：
2012-12-15
期刊：
World journal of gastrointestinal pathophysiology
影响因子：
--
作者：
Henderson, Wendy A;Shankar, Ravi;Youssef, Nader N
通讯作者：
Youssef, Nader N
影响因子：
24.5
作者：
Zhou Q;Souba WW;Croce CM;Verne GN
通讯作者：
Verne GN
影响因子：
3.4
作者：
Lipscombe, Diane;Andrade, Arturo;Allen, Summer E.
通讯作者：
Allen, Summer E.
影响因子：
3.5
作者：
Zhang, M.;Leung, F. -P.;Bian, Z. -X.
通讯作者：
Bian, Z. -X.