A HU-like protein is required for full virulence in Xanthomonas campestris pv. campestris.
A HU-like protein is required for full virulence in Xanthomonas campestris pv. campestris.
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野油菜黄单胞菌 pv. 的完全毒力需要 HU 样蛋白。
DOI：
10.1111/mpp.13128
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发表时间：
2021-12
影响因子：
4.9
通讯作者：
Lu GT
中科院分区：
文献类型：
--
作者：
Su Q;Wang XX;Leng M;Qi YH;Pang FY;Tang JL;Lu GT
细菌含有几种丰富的小DNA结合蛋白，称为类核相关蛋白(NAP)，它们对细菌类核的结构和基因调控都有贡献。虽然NAP作为全球转录调节因子的功能在模式生物大肠杆菌中已经得到了全面的研究，但它们在其他细菌中的调节功能仍然相对较少。野油菜黄单胞菌野油菜属(Campestris，Xcc)是一种革兰氏阴性细菌，在十字花科几乎所有成员中都能引起黑腐病。在以前的工作中，我们证明了一种FIS同源蛋白，我们将其命名为FIS-like蛋白(FLP)，它有助于调节XCC的毒力、III型分泌和一系列其他表型。在这里，我们研究了XC_1355的作用，它被预测编码一个属于HU家族的DNA结合蛋白，在这里被称为HU-like蛋白(HLP)。我们发现XC_1355突变降低了XCC的毒力、胞外多糖产量和细胞活力，但对胞外酶的产生和超敏反应的诱导没有影响。这些数据和转录组分析表明，HLP是一个与毒力有关的未知基因，与Xcc中的flp具有部分重叠和互补的功能，尽管这两种调控因子对参与III型分泌的基因的表达具有相反的影响。这些发现加深了我们对Xcc毒力调控的复杂调控途径的理解。HLP是一种以前未被鉴定的蛋白，它与毒力有关，与FLP相比，它对III型分泌蛋白的表达具有相反的影响。
Bacteria harbour several abundant small DNA‐binding proteins known as nucleoid‐associated proteins (NAPs) that contribute to the structure of the bacterial nucleoid as well as to gene regulation. Although the function of NAPs as global transcriptional regulators has been comprehensively studied in the model organism Escherichia coli, their regulatory functions in other bacteria remain relatively poorly understood. Xanthomonas campestris pv. campestris (Xcc) is a gram‐negative bacterium that causes black rot disease in almost all members of the crucifer family. In previous work, we demonstrated that a Fis homologue protein, which we named Fis‐like protein (Flp), contributes to the regulation of virulence, type III secretion, and a series of other phenotypes in Xcc. Here we have examined the role of XC_1355, which is predicted to encode a DNA‐binding protein belonging to the HU family herein named HU‐like protein (Hlp). We show that mutation of XC_1355 in Xcc reduces the virulence, extracellular polysaccharide production, and cell motility, but has no effect on the production of extracellular enzymes and induction of the hypersensitive response. These data together with transcriptome analysis indicate that hlp is a previously uncharacterized gene involved in virulence that has partially overlapping and complementary functions with flp in Xcc, although the two regulators have opposite effects on the expression of genes involved in type III secretion. The findings add to our understanding of the complex regulatory pathways that act to regulate virulence in Xcc. A previously uncharacterized protein, Hlp, is involved in virulence and has opposite effects on the expression of type III secretion proteins compared with Flp.
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