Coexistence of multiple genotypes of porcine epidemic diarrhea virus with novel mutant S genes in the Hubei Province of China in 2016.
Coexistence of multiple genotypes of porcine epidemic diarrhea virus with novel mutant S genes in the Hubei Province of China in 2016.
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2016年湖北省猪流行性腹泻病毒多基因型与S基因新突变共存
DOI：
10.1007/s12250-017-4021-8
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发表时间：
2017-08
影响因子：
5.5
通讯作者：
Ge XY
中科院分区：
文献类型：
--
作者：
Zeng Z;Li TT;Jin X;Peng FH;Song NH;Peng GQ;Ge XY
2010年，我国猪流行性腹泻病毒（PEDV）强毒株的出现给我国经济造成了巨大损失。此后，我国偶尔发生由PEDV变异株引起的PED大规模散发疫情。然而，不同省份PEDV的分子多样性和流行病学尚未完全了解。为了确定中国湖北省PEDV的分子多样性，我们于2016年从全省34个养殖场收集了172份PED样品，并通过靶向核衣壳（N）基因进行逆转录聚合酶链反应（RT-PCR）。74个样本被发现是PEDV阳性。我们进一步从阳性样本中鉴定了完整的S糖蛋白基因，发现了21个不同的S基因，这些S基因具有氨基酸突变。这里的PEDV分离株呈现了以前在东亚和东南亚、北美和欧洲的田间分离株中发现的大多数基因型。在S基因中发现了58个新的氨基酸突变位点，其中S1突变位点44个，S2突变位点14个。结果表明，PEDV S基因存在变异，不同基因型PEDV共存，是导致2016年湖北省PED暴发的主要原因。这项工作突出了PEDV流行病学的复杂性，并强调需要重新评估经典PEDV疫苗对新出现的变异株的效力，并开发新的疫苗以促进田间PEDV的预防和控制。
The emergence of highly virulent porcine epidemic diarrhea virus (PEDV) variants in China caused huge economic losses in 2010. Since then, large-scale sporadic outbreaks of PED caused by PEDV variants have occasionally occurred in China. However, the molecular diversity and epidemiology of PEDV in different provinces has not been completely understood. To determine the molecular diversity of PEDV in the Hubei Province of China, we collected 172 PED samples from 34 farms across the province in 2016 and performed reverse transcription polymerase chain reaction (RT-PCR) by targeting the nucleocapsid (N) gene. Seventy-four samples were found to be PEDV-positive. We further characterized the complete spike (S) glycoprotein genes from the positive samples and found 21 different S genes with amino acid mutations. The PEDV isolates here presented most of the genotypes which were found previously in field isolates in East and South-East Asia, North America, and Europe. Besides the typical Genotypes I and II, the INDEX groups were also found. Importantly, 58 new amino acids mutant sites in the S genes, including 44 sites in S1 and 14 sites in S2, were first described. Our results revealed that the S genes of PEDV showed variation and that diverse genotypes of PEDV coexisted and were responsible for the PED outbreaks in Hubei in 2016. This work highlighted the complexity of the epidemiology of PEDV and emphasized the need for reassessing the efficacy of classic PEDV vaccines against emerging variant strains and developing new vaccines to facilitate the prevention and control of PEDV in fields.
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DOI：
10.1038/nrmicro2147
发表时间：
2009-06
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Lee C
通讯作者：
Lee C
中国猪流行性腹泻病毒刺突基因检测及分子多样性
DOI：
10.3390/v5102601
发表时间：
2013-10-22
期刊：
Viruses
影响因子：
--
作者：
Chen J;Liu X;Shi D;Shi H;Zhang X;Li C;Chi Y;Feng L
通讯作者：
Feng L
影响因子：
5
作者：
Cruz DJ;Kim CJ;Shin HJ
通讯作者：
Shin HJ
影响因子：
11.8
作者：
Tian PF;Jin YL;Xing G;Qv LL;Huang YW;Zhou JY
通讯作者：
Zhou JY