High levels of sphingolipids in human breast cancer.
High levels of sphingolipids in human breast cancer.
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DOI：
10.1016/j.jss.2016.05.022
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发表时间：
2016-08
影响因子：
2.2
通讯作者：
Wakai, Toshifumi
中科院分区：
文献类型：
--
作者：
Nagahashi, Masayuki;Tsuchida, Junko;Moro, Kazuki;Hasegawa, Miki;Tatsuda, Kumiko;Woelfel, Ingrid A.;Takabe, Kazuaki;Wakai, Toshifumi
鞘磷脂，包括鞘氨醇-1-磷酸(S1P)和神经酰胺，已经成为控制肿瘤细胞生长和增殖的各个方面的关键调节分子。尽管在实验模型中已经报道了鞘磷脂在乳腺癌进展中的重要作用，但它们在人类患者中的作用尚未确定。本研究的目的是测定乳腺癌和正常乳腺组织中鞘磷脂的水平，并比较两种组织中各鞘脂水平的差异。取自12例乳腺癌患者的乳腺肿瘤和非癌组织。采用LC-ESI-MS/MS联用技术对鞘氨醇、神经酰胺及其代谢物鞘氨醇、神经鞘磷脂和单己糖神经酰胺进行了测定。肿瘤组织中S1P、神经酰胺等鞘磷脂含量显著高于正常乳腺组织。肿瘤组织与同一人正常乳腺组织中S1P水平有较强的相关性。另一方面，肿瘤和来自同一人的正常乳腺组织之间的大多数神经酰胺类物质的水平没有相关性。据我们所知，这是第一项揭示乳腺癌患者癌组织中鞘脂水平通常高于正常乳腺组织的研究。这些组织中S1P水平的相关性暗示了S1P在肿瘤与肿瘤微环境相互作用中的作用。
Sphingolipids, including sphingosine-1-phosphate (S1P) and ceramide, have emerged as key regulatory molecules that control various aspects of cell growth and proliferation in cancer. Although important roles of sphingolipids in breast cancer progression have been reported in experimental models, their roles in human patients have yet to be determined. The aim of this study is to determine the levels of sphingolipids including S1P, ceramides, and other sphingolipids, in breast cancer and normal breast tissue and to compare the difference in levels of each sphingolipid between the two tissues. Tumor and non-cancerous breast tissue were obtained from 12 patients with breast cancer. Sphingolipids including S1P, ceramides, and their metabolites of sphingosine, sphingomyelin, and monohexosylceramide were measured by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). The levels of S1P, ceramides, and other sphingolipids in the tumor were significantly higher than those in normal breast tissue. There was a relatively strong correlation in the levels of S1P between the tumor and that of normal breast tissue from the same person. On the other hand, there was no correlation in the levels of most of the ceramide species between the tumor and that of normal breast tissue from the same person. To our knowledge this is the first study to reveal that levels of sphingolipids in cancer tissue are generally higher than normal breast tissue in patients with breast cancer. The correlation of S1P levels in these tissues implicates the role of S1P in interaction between cancer and the tumor microenvironment.
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DOI：
10.4137/bcbcr.s25460
发表时间：
2015
期刊：
Breast cancer : basic and clinical research
影响因子：
--
作者：
Jin X;Mu P
通讯作者：
Mu P
DOI：
10.1126/science.1176709
发表时间：
2009-09-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hait NC;Allegood J;Maceyka M;Strub GM;Harikumar KB;Singh SK;Luo C;Marmorstein R;Kordula T;Milstien S;Spiegel S
通讯作者：
Spiegel S
影响因子：
2.7
作者：
Knapp, M.;Baranowski, M.;Musial, W.
通讯作者：
Musial, W.
影响因子：
11.2
作者：
Nagahashi M;Ramachandran S;Kim EY;Allegood JC;Rashid OM;Yamada A;Zhao R;Milstien S;Zhou H;Spiegel S;Takabe K
通讯作者：
Takabe K
影响因子：
4.7
作者：
Pyne S;Edwards J;Ohotski J;Pyne NJ
通讯作者：
Pyne NJ