Detection of Escherichia coli enteropathogens by multiplex polymerase chain reaction from children's diarrheal stools in two Caribbean-Colombian cities.
Detection of Escherichia coli enteropathogens by multiplex polymerase chain reaction from children's diarrheal stools in two Caribbean-Colombian cities.
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DOI：
10.1089/fpd.2009.0355
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发表时间：
2010-02
影响因子：
2.8
通讯作者：
Castro GY
中科院分区：
文献类型：
--
作者：
Gómez-Duarte OG;Arzuza O;Urbina D;Bai J;Guerra J;Montes O;Puello M;Mendoza K;Castro GY
急性腹泻病是发展中国家儿童发病率和死亡率的主要原因，大肠杆菌肠道病原体是重要的病原体。流行病学资料。大肠杆菌肠道病原体及其与腹泻病的关联有限，因为在资源有限的国家没有诊断测试。目的评价E.大肠杆菌肠道病原体在加勒比-哥伦比亚地区，E.应用双反应多重聚合酶链反应技术对腹泻患儿大肠杆菌临床分离株进行分析。所有E.大肠杆菌分离株也分型的单反应多重聚合酶链反应。我们发现在139株E.大肠埃希菌20株（14.4%）;大肠杆菌致病型。产肠毒素、产志贺毒素、肠聚集性、弥漫性粘附性和肠致病性E.大肠杆菌致病型的检测，其中大部分属于系统发育组A和B1，已知与肠道病原体。这是首次报道E.哥伦比亚大肠杆菌产大肠杆菌分离株，并首次报道了大肠杆菌的潜在作用。在这个地理区域儿童腹泻中的大肠杆菌。
Acute diarrheal disease is a leading cause of childhood morbidity and mortality in the developing world and Escherichia coli intestinal pathogens are important causative agents. Information on the epidemiology of E. coli intestinal pathogens and their association with diarrheal disease is limited because no diagnostic testing is available in countries with limited resources. To evaluate the prevalence of E. coli intestinal pathogens in a Caribbean–Colombian region, E. coli clinical isolates from children with diarrhea were analyzed by a recently reported two-reaction multiplex polymerase chain reaction. The phylogenetic group from all E. coli isolates was also typed by a single-reaction multiplex polymerase chain reaction. We found that among 139 E. coli strains analyzed, 20 (14.4%) corresponded to E. coli diarrheagenic pathotypes. Enterotoxigenic, shiga-toxin–producing, enteroaggregative, diffuse adherent, and enteropathogenic E. coli pathotypes were detected, and most of them belonged to the phylogenetic groups A and B1, known to be associated with intestinal pathogens. This is the first report on the molecular characterization of E. coli diarrheogenic isolates in Colombia and the first report on the potential role of E. coli in childhood diarrhea in this geographic area.
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DOI：
10.1590/s0074-02762007005000116
发表时间：
2007-11-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Bueris, Vanessa;Sircili, Marcelo Palma;Trabulsi, Luiz Rachid
通讯作者：
Trabulsi, Luiz Rachid
DOI：
10.2471/blt.06.030866
发表时间：
2007-01-01
期刊：
Bulletin of the World Health Organization: International Journal of Public Health
影响因子：
--
作者：
Forsberg, Birger Carl;Petzold, Max G;Allebeck, Peter
通讯作者：
Allebeck, Peter
影响因子：
10.7
作者：
Escobar-Páramo, P;Clermont, O;Denamur, E
通讯作者：
Denamur, E
DOI：
10.2471/blt.07.050054
发表时间：
2008-09-01
期刊：
Bulletin of the World Health Organization: International Journal of Public Health
影响因子：
--
作者：
Boschi-Pinto, Cynthia;Velebit, Lana;Shibuya, Kenji
通讯作者：
Shibuya, Kenji
影响因子：
3.2
作者：
HERZER, PJ;INOUYE, S;WHITTAM, TS
通讯作者：
WHITTAM, TS