Efferent pathways of the mouse lateral habenula.
Efferent pathways of the mouse lateral habenula.
复制标题
DOI：
10.1002/cne.23662
复制
发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Turner, Eric E.
中科院分区：
文献类型：
--
作者：
Quina, Lely A.;Tempest, Lynne;Ng, Lydia;Harris, Julie A.;Ferguson, Susan;Jhou, Thomas C.;Turner, Eric E.
外侧缰核是丘脑背侧缰核复合体的一部分。最近对LHb的研究主要集中在其向腹侧被盖区(VTA)和被盖头内侧核(RMTg)的投射，这两个区域含有GABA能神经元，通过抑制多巴胺能活动来调节奖赏预测错误。然而，在大鼠中较早的研究也发现LHb输出到下丘脑的外侧和后部、中缝正中缝、中缝背侧和背侧被盖。虽然这些研究表明LHb的内侧和外侧分支有一些不同的投射，但LHb传出的局部特异性尚不完全清楚，这些投射与脑干靶点的相对程度也是未知的。在这里，我们使用腺相关病毒介导的绿色荧光蛋白表达的顺行示踪法，结合连续的双光子断层扫描，在整个小鼠大脑的标准坐标系上映射LHb的传出，并在三维上重建LHb的传出路径。使用纤维密度的自动定量，我们表明，除了RMTG，中缝正中缝、尾侧中缝背侧和脑桥中央灰质是LHb传出的主要受体。用霍乱毒素B亚单位逆行追踪的方法，我们发现投射到下丘脑、VTA、中缝正中、中缝尾侧背侧和桥脑中央灰质的LHb神经元分布在LHb特有但有时重叠的区域。这些结果为系统研究LHb在小鼠神经回路和行为中的作用提供了解剖学基础。
The lateral habenula (LHb) is part of the habenula complex of the dorsal thalamus. Recent studies of the LHb have focused on its projections to the ventral tegmental area (VTA) and rostromedial tegmental nucleus (RMTg), which contain GABAergic neurons that mediate reward prediction error via inhibition of dopaminergic activity. However, older studies in the rat have also identified LHb outputs to the lateral and posterior hypothalamus, median raphe, dorsal raphe, and dorsal tegmentum. Although these studies have shown that the medial and lateral divisions of the LHb have somewhat distinct projections, the topographic specificity of LHb efferents is not completely understood, and the relative extent of these projections to brainstem targets is unknown. Here we have used anterograde tracing with adeno-associated virus mediated expression of green fluorescent protein, combined with serial two-photon tomography, to map the efferents of the LHb on a standard coordinate system for the entire mouse brain, and reconstruct the efferent pathways of the LHb in three dimensions. Using automated quantitation of fiber density, we show that in addition to the RMTg, the median raphe, caudal dorsal raphe, and pontine central gray are major recipients of LHb efferents. Using retrograde tract tracing with cholera toxin subunit B, we show that LHb neurons projecting to the hypothalamus, VTA, median raphe, and caudal dorsal raphe, and pontine central gray reside in characteristic, but sometimes overlapping regions of the LHb. Together these results provide the anatomical basis for systematic studies of LHb function in neural circuits and behavior in mice.
登录
查看更多内容
影响因子：
25
作者：
通讯作者：
--
影响因子：
3
作者：
Lau, Christopher;Ng, Lydia;Hawrylycz, Mike
通讯作者：
Hawrylycz, Mike
影响因子：
16.2
作者：
Jhou, Thomas C.;Fields, Howard L.;Baxter, Mark G.;Saper, Clifford B.;Holland, Peter C.
通讯作者：
Holland, Peter C.
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
2.9
作者：
AGHAJANIAN, GK;WANG, RY
通讯作者：
WANG, RY