Isolation and characterization of cellulose-decomposing bacteria inhabiting sawdust and coffee residue composts.
Isolation and characterization of cellulose-decomposing bacteria inhabiting sawdust and coffee residue composts.
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DOI：
10.1264/jsme2.me11299
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发表时间：
2012
影响因子：
2.2
通讯作者：
Kouno K
中科院分区：
文献类型：
--
作者：
Fathallh Eida M;Nagaoka T;Wasaki J;Kouno K
澄清与有机材料分解相关的微生物的身份和酶活性，预计将有助于评估和改进堆肥过程。在这项研究中，我们研究了从木屑堆肥（SDC）和咖啡渣堆肥（CRC）分离的细菌的纤维素和半纤维素分解能力。使用含有天青素交联（AZCL）HE-纤维素的Dubos矿物盐琼脂分离纤维素分解细菌。根据16 S rRNA基因序列分析进行细菌鉴定，并使用不溶性AZCL连接的底物表征纤维素酶、木聚糖酶、β-葡聚糖酶、甘露聚糖酶和蛋白酶活性。11株分离自SDC，10株分离自CRC。DNA序列分析表明，SDC和CRC的分离物分别属于链霉菌属、微双孢属和类芽孢杆菌属，以及链霉菌属、微双孢属和科恩氏菌属。微双孢菌是两种堆肥类型中最具优势的菌属。除两株缺乏甘露聚糖酶活性外，其余菌株均表现出纤维素酶、木聚糖酶、β-葡聚糖酶和甘露聚糖酶活性。根据水解区直径与菌落直径比值的酶活性，认为SDC中的Microbispora（SDCB 8、SDCB 9）和Paenibacillus（SDCB 10、SDCB 11）以及CRC中的Microbispora（CRCB 2、CRCB 6）和Cohnella（CRCB 9、CRCB 10）有助于堆肥过程中高效的纤维素和半纤维素分解过程。
Clarifying the identity and enzymatic activities of microorganisms associated with the decomposition of organic materials is expected to contribute to the evaluation and improvement of composting processes. In this study, we examined the cellulolytic and hemicellulolytic abilities of bacteria isolated from sawdust compost (SDC) and coffee residue compost (CRC). Cellulolytic bacteria were isolated using Dubos mineral salt agar containing azurine cross-linked (AZCL) HE-cellulose. Bacterial identification was performed based on the sequence analysis of 16S rRNA genes, and cellulase, xylanase, β-glucanase, mannanase, and protease activities were characterized using insoluble AZCL-linked substrates. Eleven isolates were obtained from SDC and 10 isolates from CRC. DNA analysis indicated that the isolates from SDC and CRC belonged to the genera Streptomyces, Microbispora, and Paenibacillus, and the genera Streptomyces, Microbispora, and Cohnella, respectively. Microbispora was the most dominant genus in both compost types. All isolates, with the exception of two isolates lacking mannanase activity, showed cellulase, xylanase, β-glucanase, and mannanase activities. Based on enzyme activities expressed as the ratio of hydrolysis zone diameter to colony diameter, it was suggested that the species of Microbispora (SDCB8, SDCB9) and Paenibacillus (SDCB10, SDCB11) in SDC and Microbispora (CRCB2, CRCB6) and Cohnella (CRCB9, CRCB10) in CRC contribute to efficient cellulolytic and hemicellulolytic processes during composting.
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影响因子：
4.2
作者：
Chidthaisong, A;Conrad, R
通讯作者：
Conrad, R
影响因子：
3.2
作者：
Dubos, RJ
通讯作者：
Dubos, RJ
影响因子：
2.8
作者：
Luo Xuesong;Wang, Zhang;Fang, Chengxiang
通讯作者：
Fang, Chengxiang
DOI：
10.1196/annals.1419.002
发表时间：
2008-01-01
期刊：
INCREDIBLE ANAEROBES: FROM PHYSIOLOGY TO GENOMICS TO FUELS
影响因子：
--
作者：
Doi, Roy H.
通讯作者：
Doi, Roy H.
影响因子：
2.8
作者：
Hong, TY;Cheng, CW;Meng, MS
通讯作者：
Meng, MS