A Model for Stress Fiber Realignment Caused by Cytoskeletal Fluidization During Cyclic Stretching.
A Model for Stress Fiber Realignment Caused by Cytoskeletal Fluidization During Cyclic Stretching.
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DOI：
10.1007/s12195-010-0152-9
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发表时间：
2011-03-01
影响因子：
2.8
通讯作者：
Stamenovic, Dimitrije
中科院分区：
文献类型：
--
作者：
Pirentis, Athanassios P.;Peruski, Elizabeth;Iordan, Andreea L.;Stamenovic, Dimitrije
关键词：
单轴循环基质拉伸导致细胞骨架组织的协同变化，使得应力纤维（SF）重新排列远离拉伸方向。最近的实验表明，短暂的短暂拉伸迅速消融细胞的收缩应力通过细胞骨架流化，然后通过重新凝固的方式缓慢的应力恢复。这反过来又表明，流体化，再固化和SF重新排列可能在拉伸过程中联系在一起。我们提出了一个数学模型，模拟流化和再固化对细胞骨架收缩应力的影响，以研究这些现象如何影响细胞骨架的重新排列响应于纯单轴拉伸的基板。该模型包括在端点处锚定到弹性基底的单个弹性SF。采用全球稳定性公约，该模型预测，在响应重复的拉伸-非拉伸周期，SF倾向于在垂直于拉伸的方向上重新排列，与文献中的数据一致。该模型被用来开发一个计算方案，用于预测在拉伸过程中细胞取向和极性的变化，以及它们如何与细胞骨架组织中的潜在变化相关。我们的结论是，细胞骨架收缩应力的流体化和随后的应力恢复的手段，通过resolidification耗尽可能发挥关键作用，在重组细胞骨架SFs单轴拉伸的基板。
Uniaxial cyclic substrate stretching results in a concerted change of cytoskeletal organization such that stress fibers (SFs) realign away from the direction of stretching. Recent experiments revealed that brief transient stretch promptly ablates cellular contractile stress by means of cytoskeletal fluidization, followed by a slow stress recovery by means of resolidification. This, in turn, suggests that fluidization, resolidification and SF realignment may be linked together during stretching. We propose a mathematical model that simulates the effects of fluidization and resolidification on cytoskeletal contractile stress in order to investigate how these phenomena affect cytoskeletal realignment in response to pure uniaxial stretching of the substrate. The model comprises of individual elastic SFs anchored at the endpoints to an elastic substrate. Employing the global stability convention, the model predicts that in response to repeated stretch–unstretch cycles, SFs tend to realign in the direction perpendicular to stretching, consistent with data from the literature. The model is used to develop a computational scheme for predicting changes in cell orientation and polarity during stretching and how they relate to the underlying alterations in the cytoskeletal organization. We conclude that depletion of cytoskeletal contractile stress by means of fluidization and subsequent stress recovery by means of resolidification may play a key role in reorganization of cytoskeletal SFs in response to uniaxial stretching of the substrate.
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作者：
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通讯作者：
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影响因子：
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