Identification of serum miR-1915-3p and miR-455-3p as biomarkers for breast cancer.
Identification of serum miR-1915-3p and miR-455-3p as biomarkers for breast cancer.
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鉴定血清 miR-1915-3p 和 miR455-3p 作为乳腺癌生物标志物
DOI：
10.1371/journal.pone.0200716
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Luo Y
中科院分区：
文献类型：
--
作者：
Guo J;Liu C;Wang W;Liu Y;He H;Chen C;Xiang R;Luo Y
乳腺癌是世界范围内女性最严重的恶性疾病之一。血清微小RNA（microRNAs，miRNAs）具有高度敏感性和特异性的特点，作为肿瘤疾病的潜在生物标志物，近年来受到越来越多的关注。在这项研究中，194名乳腺癌患者在手术前收集了血清样本，并根据他们的诊断信息被纳入不同的组。为了寻找乳腺癌诊断生物标志物，通过微阵列筛选健康志愿者和不同临床阶段的乳腺癌患者的合并样本中的血清miRNAs。在诊断和预测集中的每个个体患者的血清样品中进一步验证miRNA。与健康志愿者相比，乳腺癌患者血清中miR-1915- 3 p水平显著上调，miR-455- 3 p水平显著下调。此外，浸润性癌或淋巴结转移患者血清miR-1915- 3 p水平高于原位癌或无淋巴结转移患者，而血清miR-455- 3 p水平低于原位癌或无淋巴结转移患者。ROC分析提示miR-1915- 3 p和miR-455- 3 p有可能成为乳腺癌血清学诊断和预测的生物标志物。miR-1915- 3 p在某些人乳腺癌细胞中过表达。体外功能实验表明，miR-1915- 3 p能增强细胞的增殖和迁移能力。miR-1915- 3 p的过表达抑制了靶基因DUSP 3并激活了ERK 1/2。总的来说，这项研究提供了一个新的见解，即miR-1915- 3 p可能在乳腺癌的发展中发挥作用，血清miR-1915- 3 p和miR-455- 3 p可以作为乳腺癌的诊断和预测生物标志物。
Breast cancer is one of the most malignant diseases in women worldwide. Serum microRNAs (miRNAs), with the characteristics of high sensitivity and specificity, have recently attracted more attentions to serve as potential biomarkers for tumor diseases. In this study, 194 breast cancer patients’ serum samples were collected before surgery and enrolled into different groups based on their diagnostic information. To search for breast cancer diagnostic biomarkers, serum miRNAs were screened by microarray in pooled samples of healthy volunteers and breast cancer patients in different clinical stages. The miRNAs were further verified in each individual patient’s serum samples in diagnostic and predictive sets. The serum level of miR-1915-3p was upregulated and miR-455-3p was downregulated significantly in breast cancer patients compared with healthy volunteers. Furthermore, the patients with infiltrating carcinoma or lymph node metastasis had a higher serum level of miR-1915-3p and lower serum level of miR-455-3p than patients with the carcinoma in situ or patients without lymph node metastasis. ROC analysis suggested that miR-1915-3p and miR-455-3p had the potential as a promising serum diagnostic and predictive biomarkers of breast cancer. miR-1915-3p was over-expressed in certain human breast cancer cells. Functional experiments in vitro showed that miR-1915-3p enhanced cell proliferative and migrational abilities. Overexpression of miR-1915-3p repressed target gene DUSP3 and activated ERK1/2. Collectively, this study provided a new insight that miR-1915-3p might play a role in the development of breast cancer and that serum miR-1915-3p and miR-455-3p could serve as diagnostic and predictive biomarkers for breast cancer.
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影响因子：
5.1
作者：
Wang, Wei;Luo, Yun-ping
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1.2
作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.0804549105
发表时间：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Zhu M;Huang Z;Zhu D;Zhou X;Shan X;Qi LW;Wu L;Cheng W;Zhu J;Zhang L;Zhang H;Chen Y;Zhu W;Wang T;Liu P
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