Combining portable Raman probes with nanotubes for theranostic applications.
Combining portable Raman probes with nanotubes for theranostic applications.
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DOI：
10.7150/thno/v01p0310
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发表时间：
2011
期刊：
影响因子：
12.4
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Bhirde AA;Liu G;Jin A;Iglesias-Bartolome R;Sousa AA;Leapman RD;Gutkind JS;Lee S;Chen X
最近，便携式拉曼探测器随着各种应用的出现而出现，包括碳纳米管(CNT)的表征。分散在水中的碳纳米管在药物/基因传递载体、光热疗法和光声成像等生物医学领域具有广阔的应用前景。在这项研究中，我们报道了使用便携式拉曼探头在2D单层癌细胞和3D球体中同时检测和照射碳纳米管。便携式拉曼仪有两种用途：作为碳纳米管探测器和作为照射激光光源。单壁碳纳米管(SWCNTs)和多壁碳纳米管(MWCNTs)被脂类聚合物(LP)涂层分散在水中，在缓冲液和细胞介质中都形成了高度稳定的分散体。用原子力显微镜、透射电子显微镜、动态光散射、傅里叶变换红外光谱和拉曼光谱对包覆后的单壁碳纳米管和多壁碳纳米管水分散体进行了表征。共聚焦显微镜观察细胞对脂蛋白分散的SWCNTs和MWCNTs的摄取，Z-STACK荧光共聚焦成像发现异硫氰酸荧光素(FITC)-纳米管结合物可被卵巢癌细胞内化。用细胞活力四甲基偶氮唑盐比色法测定了SWCNTs和MWCNTs的生物相容性，结果表明纳米管分散体在所测试的剂量下对卵巢癌细胞的增殖没有影响。使用便携式拉曼探头，同时检测经SWCNTs和MWCNTs处理的卵巢癌细胞，并将其照射在2D层癌细胞和3D环境中。激光照射后进行的凋亡末端脱氧核苷酸转移酶dUTP缺口末端标记(TUNEL)分析证实，只有在纳米管分散体存在的情况下，细胞才会死亡。我们首次展示了单壁碳纳米管和多壁碳纳米管都可以用简单的手持式拉曼仪器选择性地照射并在癌细胞中检测到。这种方法可能被用于治疗包括癌症在内的各种疾病。
Recently portable Raman probes have emerged along with a variety of applications, including carbon nanotube (CNT) characterization. Aqueous dispersed CNTs have shown promise for biomedical applications such as drug/gene delivery vectors, photo-thermal therapy, and photoacoustic imaging. In this study we report the simultaneous detection and irradiation of carbon nanotubes in 2D monolayers of cancer cells and in 3D spheroids using a portable Raman probe. A portable handheld Raman instrument was utilized for dual purposes: as a CNT detector and as an irradiating laser source. Single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) were dispersed aqueously using a lipid-polymer (LP) coating, which formed highly stable dispersions both in buffer and cell media. The LP coated SWCNT and MWCNT aqueous dispersions were characterized by atomic force microscopy, transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy and Raman spectroscopy. The cellular uptake of the LP-dispersed SWCNTs and MWCNTs was observed using confocal microscopy, and fluorescein isothiocyanate (FITC)-nanotube conjugates were found to be internalized by ovarian cancer cells by using Z-stack fluorescence confocal imaging. Biocompatibility of SWCNTs and MWCNTs was assessed using a cell viability MTT assay, which showed that the nanotube dispersions did not hinder the proliferation of ovarian cancer cells at the dosage tested. Ovarian cancer cells treated with SWCNTs and MWCNTs were simultaneously detected and irradiated live in 2D layers of cancer cells and in 3D environments using the portable Raman probe. An apoptotic terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay carried out after laser irradiation confirmed that cell death occurred only in the presence of nanotube dispersions. We show for the first time that both SWCNTs and MWCNTs can be selectively irradiated and detected in cancer cells using a simple handheld Raman instrument. This approach could potentially be used to treat various diseases, including cancer.
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影响因子：
3.5
作者：
Kotova S;Prasad K;Smith PD;Lafer EM;Nossal R;Jin AJ
通讯作者：
Jin AJ
影响因子：
17.1
作者：
Bhirde, Ashwin A.;Patel, Vyomesh;Gavard, Julie;Zhang, Guofeng;Sousa, Alioscka A.;Masedunskas, Andrius;Leapman, Richard D.;Weigert, Roberto;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
DOI：
10.1098/rsta.2010.0075
发表时间：
2010-07-13
影响因子：
5
作者：
Jehlicka, J.;Edwards, H. G. M.;Culka, A.
通讯作者：
Culka, A.
DOI：
10.1038/nrc2644
发表时间：
2009-06
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
DOI：
10.2217/nnm.10.90
发表时间：
2010-12
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Bhirde AA;Patel S;Sousa AA;Patel V;Molinolo AA;Ji Y;Leapman RD;Gutkind JS;Rusling JF
通讯作者：
Rusling JF