Distribution and clearance of PEG-single-walled carbon nanotube cancer drug delivery vehicles in mice.
Distribution and clearance of PEG-single-walled carbon nanotube cancer drug delivery vehicles in mice.
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DOI：
10.2217/nnm.10.90
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Rusling JF
中科院分区：
文献类型：
--
作者：
Bhirde AA;Patel S;Sousa AA;Patel V;Molinolo AA;Ji Y;Leapman RD;Gutkind JS;Rusling JF
目的研究聚乙二醇（PEG）基化单壁碳纳米管（SWCNTs）作为抗癌药物顺铂在小鼠体内的分布和清除。将PEG层附着在SWCNTs上，分散在水介质中，并使用动态光散射、扫描透射电子显微镜和拉曼光谱对其进行表征。采用Annexin-V法评估其体外细胞毒性，采用组织染色和拉曼光谱法研究其在小鼠体内的分布和清除途径。研究peg - swcnts -顺铂对小鼠肿瘤生长的抑制作用。与对照组相比，PEG-SWCNTs有效地分散在水介质中，并且不诱导体外细胞凋亡。在静脉注射纳米管生物偶联物的小鼠的几个重要器官组织中，苏木精和伊红染色以及SWCNTs的拉曼条带显示，与PEG-SWCNTs相比，对照SWCNTs以大聚集体形式存在于肺组织中，而PEG-SWCNTs几乎没有或没有聚集体。粪便中特征性的swcnts拉曼带显示存在胆道或肾脏排泄途径。用z -对比扫描透射电子显微镜观察顺铂在生物偶联物上的附着。结合靶向配体EGF的peg - swcnts -顺铂成功抑制小鼠头颈部肿瘤异种移植物的生长。与对照SWCNTs相反，PEG-SWCNTs形成更高度分散的递送载体，当同时装载顺铂和EGF时，可抑制鳞状细胞肿瘤的生长。
To study the distribution and clearance of polyethylene glycol (PEG)-ylated single-walled carbon nanotube (SWCNTs) as drug delivery vehicles for the anticancer drug cisplatin in mice. PEG layers were attached to SWCNTs and dispersed in aqueous media and characterized using dynamic light scattering, scanning transmission electron microscopy and Raman spectroscopy. Cytotoxicity was assessed in vitro using Annexin-V assay, and the distribution and clearance pathways in mice were studied by histological staining and Raman spectroscopy. Efficacy of PEG-SWCNT–cisplatin for tumor growth inhibition was studied in mice. PEG-SWCNTs were efficiently dispersed in aqueous media compared with controls, and did not induce apoptosis in vitro. Hematoxylin and eosin staining, and Raman bands for SWCNTs in tissues from several vital organs from mice injected intravenously with nanotube bioconjugates revealed that control SWCNTs were lodged in lung tissue as large aggregates compared with the PEG-SWCNTs, which showed little or no accumulation. Characteristic SWCNT Raman bands in feces revealed the presence of bilary or renal excretion routes. Attachment of cisplatin on bioconjugates was visualized with Z-contrast scanning transmission electron microscopy. PEG-SWCNT–cisplatin with the attached targeting ligand EGF successfully inhibited growth of head and neck tumor xenografts in mice. PEG-SWCNTs, as opposed to control SWCNTs, form more highly dispersed delivery vehicles that, when loaded with both cisplatin and EGF, inhibit growth of squamous cell tumors.
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影响因子：
3.7
作者：
Li, Y;Kwon, GS
通讯作者：
Kwon, GS
影响因子：
17.1
作者：
Bhirde, Ashwin A.;Patel, Vyomesh;Gavard, Julie;Zhang, Guofeng;Sousa, Alioscka A.;Masedunskas, Andrius;Leapman, Richard D.;Weigert, Roberto;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
15
作者：
Kam, NWS;Dai, HJ
通讯作者：
Dai, HJ
影响因子：
16.6
作者：
Liu, Y;Wu, DC;Leong, KW
通讯作者：
Leong, KW
DOI：
10.2217/nnm.09.56
发表时间：
2009-10
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Bhirde AA;Sousa AA;Patel V;Azari AA;Gutkind JS;Leapman RD;Rusling JF
通讯作者：
Rusling JF