Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation.
Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation.
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DOI：
10.1038/srep33548
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发表时间：
2016-09-15
影响因子：
4.6
通讯作者：
Fukai S
中科院分区：
文献类型：
--
作者：
Kimura S;Yamashita M;Yamakami-Kimura M;Sato Y;Yamagata A;Kobashigawa Y;Inagaki F;Amada T;Hase K;Iwanaga T;Ohno H;Fukai S
隧道纳米管（TNT）是一种用于细胞间通讯的结构，是介导各种信号分子和细胞成分运输的薄膜突起。 M-Sec 具有强大的膜变形能力，并与 Ral/exocyst 复合物配合诱导 TNT 形成。在这里，我们发现 M-Sec 的 N 端多元区域直接结合磷脂酰肌醇 (4,5)-二磷酸，在 TNT 形成的初始阶段定位到质膜。我们进一步报道了 M-Sec 的晶体结构，它由排列成直棒状的螺旋束组成，类似于膜束缚复合物亚基。 M-Sec 与活性 RalA 相互作用以延伸质膜突起，需要 C 端结构域中带正电荷的表面。我们的结果表明，膜相关的 M-Sec 招募活性 RalA，指导外囊复合物形成 TNT。
The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.
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影响因子：
64.5
作者：
Chevrier N;Mertins P;Artyomov MN;Shalek AK;Iannacone M;Ciaccio MF;Gat-Viks I;Tonti E;DeGrace MM;Clauser KR;Garber M;Eisenhaure TM;Yosef N;Robinson J;Sutton A;Andersen MS;Root DE;von Andrian U;Jones RB;Park H;Carr SA;Regev A;Amit I;Hacohen N
通讯作者：
Hacohen N
影响因子：
5.6
作者：
Hamburger, ZA;Hamburger, AE;Weis, WI
通讯作者：
Weis, WI
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
11.4
作者：
Frankel, P;Aronheim, A;Marshall, CJ
通讯作者：
Marshall, CJ
影响因子：
16.8
作者：
Dong, G;Hutagalung, AH;Reinisch, KM
通讯作者：
Reinisch, KM