Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions.
Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions.
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DOI：
10.3389/fcimb.2014.00001
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发表时间：
2014
影响因子：
5.7
通讯作者：
Call DR
中科院分区：
文献类型：
--
作者：
Nydam SD;Shah DH;Call DR
副溶血弧菌是一种新兴的细菌病原体，能够引起炎症性胃肠炎、伤口感染和败血症。作为一种食源性疾病，感染最常与食用生的或未煮熟的海鲜（尤其是贝类）有关。它是美国弧菌相关食源性疾病的主要原因，也是日本食源性疾病的主要原因。它的两条染色体中较大的一条含有一组编码 III 型节段系统 1 (T3SS1) 的基因，该毒力因子存在于所有副溶血性弧菌菌株中，与耶尔森氏菌 ysc T3SS 相似。 T3SS1 将效应蛋白易位到真核细胞中，诱导细胞生理学变化并调节宿主与病原体的相互作用。 T3SS1 还负责对几种不同培养细胞系的细胞毒性以及小鼠模型的死亡率。在此，我们使用 RNA-seq 来获得副溶血弧菌在诱导[在转录调节因子 exsA 的反式表达中在 Dulbecco's Modified Eagle Medium (DMEM) 培养基中生长]或抑制 T3SS1 表达（在 LB-S 培养基中生长，在反式 exsD 表达中）以及随时间感染 HeLa 细胞的条件下的全局转录组模式。比较转录组分析证明了诱导条件下表达模式的显着差异，并且还用于生成 HeLa 细胞感染期间副溶血弧菌的表达谱。此外，我们还发现了几个与 T3SS1 表达相关的新基因，可能值得进一步研究。
Vibrio parahaemolyticus is an emerging bacterial pathogen capable of causing inflammatory gastroenteritis, wound infections, and septicemia. As a food-borne illness, infection is most frequently associated with the consumption of raw or undercooked seafood, particularly shellfish. It is the primary cause of Vibrio-associated food-borne illness in the United States and the leading cause of food-borne illness in Japan. The larger of its two chromosomes harbors a set of genes encoding type III section system 1 (T3SS1), a virulence factor present in all V. parahaemolyticus strains that is similar to the Yersinia ysc T3SS. T3SS1 translocates effector proteins into eukaryotic cells where they induce changes to cellular physiology and modulate host-pathogen interactions. T3SS1 is also responsible for cytotoxicity toward several different cultured cell lines as well as mortality in a mouse model. Herein we used RNA-seq to obtain global transcriptome patterns of V. parahaemolyticus under conditions that either induce [growth in Dulbecco's Modified Eagle Medium (DMEM) media, in trans expression of transcriptional regulator exsA] or repress T3SS1 expression (growth in LB-S media, in trans exsD expression) and during infection of HeLa cells over time. Comparative transcriptomic analysis demonstrated notable differences in the expression patterns under inducing conditions and was also used to generate an expression profile of V. parahaemolyticus during infection of HeLa cells. In addition, we identified several new genes that are associated with T3SS1 expression and may warrant further study.
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影响因子：
3.6
作者：
Burdette DL;Seemann J;Orth K
通讯作者：
Orth K
影响因子：
12.3
作者：
Giannoukos G;Ciulla DM;Huang K;Haas BJ;Izard J;Levin JZ;Livny J;Earl AM;Gevers D;Ward DV;Nusbaum C;Birren BW;Gnirke A
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Gnirke A
影响因子：
2.2
作者：
Chen, CY;Nace, GW;Irwin, PL
通讯作者：
Irwin, PL
DOI：
10.1016/j.biocel.2012.03.002
发表时间：
2012-06-01
影响因子：
4
作者：
Aguilera, Laura;Ferreira, Elaine;Baldoma, Laura
通讯作者：
Baldoma, Laura
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y