Microbial Communities of Hydrothermal Guaymas Basin Surficial Sediment Profiled at 2 Millimeter-Scale Resolution.
Microbial Communities of Hydrothermal Guaymas Basin Surficial Sediment Profiled at 2 Millimeter-Scale Resolution.
复制标题
DOI：
10.3389/fmicb.2021.710881
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
Teske A
中科院分区：
文献类型：
--
作者：
Engelen B;Nguyen T;Heyerhoff B;Kalenborn S;Sydow K;Tabai H;Peterson RN;Wegener G;Teske A
Guaymas盆地表层热液沉积物中存在复杂的微生物群落，其中氧化和还原性氮、硫和碳循环种群和过程重叠共存。本文采用变性梯度凝胶电泳（DGGE）技术，在2毫米尺度上分析了瓜伊马斯盆地热液沉积物岩心的微生物群落特征，以观察优势细菌和古细菌的快速变化。细菌16S rRNA基因扩增子的DGGE分析鉴定出了自由生活的和合养的三角洲变形菌硫酸盐还原菌、发酵细胞噬菌、热柔菌、氨青菌和未培养的沉积物分支。DGGE模式显示了一个逐渐变化的群落结构，2毫米尺度的微小变化累积到4厘米沉积物层内显著变化的种群。功能基因DGGE分析根据甲基辅酶M还原酶基因鉴定出厌氧甲烷氧化古菌（ANME），根据细菌和古菌氨单加氧酶基因鉴定出Betaproteobacteria和Thaumarchaeota成员。瓜伊马斯盆地热液沉积物中好氧、硝化、硫酸盐还原和发酵细菌和古细菌（包括嗜热菌）在表层沉积物中共存和重叠的栖息地范围与维持高度复杂微生物群落的动态氧化还原和热梯度一致。
The surficial hydrothermal sediments of Guaymas Basin harbor complex microbial communities where oxidative and reductive nitrogen, sulfur, and carbon-cycling populations and processes overlap and coexist. Here, we resolve microbial community profiles in hydrothermal sediment cores of Guaymas Basin on a scale of 2 millimeters, using Denaturing Gradient Gel Electrophoresis (DGGE) to visualize the rapid downcore changes among dominant bacteria and archaea. DGGE analysis of bacterial 16S rRNA gene amplicons identified free-living and syntrophic deltaproteobacterial sulfate-reducing bacteria, fermentative Cytophagales, members of the Chloroflexi (Thermoflexia), Aminicenantes, and uncultured sediment clades. The DGGE pattern indicates a gradually changing downcore community structure where small changes on a 2-millimeter scale accumulate to significantly changing populations within the top 4 cm sediment layer. Functional gene DGGE analyses identified anaerobic methane-oxidizing archaea (ANME) based on methyl-coenzyme M reductase genes, and members of the Betaproteobacteria and Thaumarchaeota based on bacterial and archaeal ammonia monooxygenase genes, respectively. The co-existence and overlapping habitat range of aerobic, nitrifying, sulfate-reducing and fermentative bacteria and archaea, including thermophiles, in the surficial sediments is consistent with dynamic redox and thermal gradients that sustain highly complex microbial communities in the hydrothermal sediments of Guaymas Basin.
登录
查看更多内容
影响因子：
5.2
作者：
Buckley, Andrew;MacGregor, Barbara;Teske, Andreas
通讯作者：
Teske, Andreas
DOI：
10.1099/ijs.0.055855-0
发表时间：
2014-06-01
影响因子：
2.8
作者：
Dodsworth, Jeremy A.;Gevorkian, Jonathan;Hedlund, Brian P.
通讯作者：
Hedlund, Brian P.
影响因子：
2.9
作者：
Kadnikov, Vitaly V.;Mardanov, Andrey V.;Ravin, Nikolai V.
通讯作者：
Ravin, Nikolai V.
DOI：
10.1073/pnas.0506625102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Francis, CA;Roberts, KJ;Oakley, BB
通讯作者：
Oakley, BB
影响因子：
5.2
作者：
Garcia-Lopez, Marina;Meier-Kolthoff, Jan P.;Goeker, Markus
通讯作者：
Goeker, Markus