Effect of Freeze-Thaw on a Midtemperate Soil Bacterial Community and the Correlation Network of Its Members.
Effect of Freeze-Thaw on a Midtemperate Soil Bacterial Community and the Correlation Network of Its Members.
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冻融对中温带土壤细菌群落及其成员相关网络的影响
DOI：
10.1155/2018/8412429
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发表时间：
2018
影响因子：
--
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Juan Y;Jiang N;Tian L;Chen X;Sun W;Chen L
冻融事件会影响土壤的功能。然而，FTs对土壤细菌群落的总体影响，特别是在温带地区，仍不清楚。在这项研究中，从中国东北的一个中温带地区收集了土壤样品，然后设计了三个培养试验，分别在冻结温度为- 15、- 9和- 3°C时设计了不同的FT振幅（即分别在冻结温度为- 15、- 9和- 3°C时）、FT循环频率（即分别在1、6和15次FT循环下）和土壤含水量（SWC）值（即分别在10%和30% SWC下）。对16S rRNA基因扩增子进行了高通量测序，并根据这些数据进一步预测了功能谱，此外还检查了大量微生物特性。数据分析表明，首先，FT振幅显著影响微生物的总体特性和细菌群落（组成和功能）；某些类群对这三个振幅表现出非线性响应。其次，与单一FTC相比，多次FT循环对细菌功能能力的影响很小，尽管多次FT循环后总体微生物特性发生了显著变化。此外，细菌对FTs的反应受到SWC的影响，表现为细菌群落结构（组成和功能）的显著差异以及酶活性的相反趋势。最后，RDA图和相关网络汇集了三个测试中所有土壤样品的数据，并表明细菌响应轨迹发生了变化，因为在FT过程中，一些物种主要受到其他物种（即生物环境）的影响。
Freeze-thaw (FT) events can influence soil functions. However, the overall impact of FTs on soil bacterial communities, especially in temperate regions, remains unclear. In this study, soil samples were collected from a midtemperate region in the northeast of China, and three incubation tests were then designed with varied FT amplitudes (i.e., at a freezing temperature of −15, −9, and −3°C, respectively), frequencies of FT cycles (i.e., under one, six, and 15 FT cycles, respectively) and soil water content (SWC) values (i.e., at 10 and 30% SWC, respectively). High-throughput sequencing of 16S rRNA gene amplicons was performed and the functional profile was further predicted based on these data, in addition to examinations of bulk microbial properties. Data analyses suggested that, first of all, the FT amplitude significantly influenced the bulk microbial properties and bacterial community (composition and function); certain taxa showed a nonlinear response to the three amplitudes. Next, compared to a single FTC, multiple FT cycles had only minor effects on the bacterial functional capabilities, although the bulk microbial properties changed significantly after multiple FT cycles. In addition, the bacterial response to FTs was influenced by the SWC, characterized by the significantly different bacterial community structures (composition and function) and the opposite trends of enzyme activities. Finally, RDA plots and a correlation network assembled data from all soil samples across the three tests and suggested that bacterial response trajectories changed because some species were influenced mainly by other species (i.e., biotic environment) during FT processes.
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影响因子：
9.7
作者：
Koponen, Hannu T.;Jaakkola, Tuula;Martikainen, Pertti J.
通讯作者：
Martikainen, Pertti J.
影响因子：
0.5
作者：
Korshunova, T. Yu.;Mukhamatdyarova, S. R.;Loginov, O. N.
通讯作者：
Loginov, O. N.
影响因子：
4.8
作者：
Cederlund, Harald;Wessen, Ella;Hallin, Sara
通讯作者：
Hallin, Sara
影响因子：
5.9
作者：
FAITH, DP
通讯作者：
FAITH, DP
影响因子：
9.7
作者：
Herrmann, A;Witter, E
通讯作者：
Witter, E