ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery.
ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery.
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ODA16通过与flagellar内运输机械的相互作用来帮助轴突外排动力蛋白组装。
DOI：
10.1083/jcb.200802025
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发表时间：
2008-10-20
期刊：
影响因子：
--
通讯作者：
Mitchell DR
中科院分区：
文献类型：
--
作者：
Ahmed NT;Gao C;Lucker BF;Cole DG;Mitchell DR
Formation of flagellar outer dynein arms in Chlamydomonas reinhardtii requires the ODA16 protein at a previously uncharacterized assembly step. Here, we show that dynein extracted from wild-type axonemes can rebind to oda16 axonemes in vitro, and dynein in oda16 cytoplasmic extracts can bind to docking sites on pf28 (oda) axonemes, which is consistent with a role for ODA16 in dynein transport, rather than subunit preassembly or binding site formation. ODA16 localization resembles that seen for intraflagellar transport (IFT) proteins, and flagellar abundance of ODA16 depends on IFT. Yeast two-hybrid analysis with mammalian homologues identified an IFT complex B subunit, IFT46, as a directly interacting partner of ODA16. Interaction between Chlamydomonas ODA16 and IFT46 was confirmed through in vitro pull-down assays and coimmunoprecipitation from flagellar extracts. ODA16 appears to function as a cargo-specific adaptor between IFT particles and outer row dynein needed for efficient dynein transport into the flagellar compartment.
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影响因子：
9.2
作者：
Pedersen, LB;Gelmer, S;Rosenbaum, JL
通讯作者：
Rosenbaum, JL
一个缺乏外动臂的运动衣原体鞭毛突变体。
影响因子：
7.8
作者：
Mitchell, D R;Rosenbaum, J L
通讯作者：
Rosenbaum, J L
影响因子：
--
作者：
MITCHELL, DR;ROSENBAUM, JL
通讯作者：
ROSENBAUM, JL
影响因子：
64.8
作者：
IRVING, M;ALLEN, TS;GOLDMAN, YE
通讯作者：
GOLDMAN, YE
衣原体插入突变体的表征，该突变体破坏了鞭毛中心对微管相关结构。
影响因子：
7.8
作者：
Mitchell, D R;Sale, W S
通讯作者：
Sale, W S