Identification of D-arabinan-degrading enzymes in mycobacteria.
Identification of D-arabinan-degrading enzymes in mycobacteria.
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DOI：
10.1038/s41467-023-37839-5
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发表时间：
2023-04-19
影响因子：
16.6
通讯作者：
Moynihan, Patrick J.
中科院分区：
文献类型：
--
作者：
Al-Jourani, Omar;Benedict, Samuel T.;Ross, Jennifer;Layton, Abigail J.;van der Peet, Phillip;Marando, Victoria M.;Bailey, Nicholas P.;Heunis, Tiaan;Manion, Joseph;Mensitieri, Francesca;Franklin, Aaron;Abellon-Ruiz, Javier;Oram, Sophia L.;Parsons, Lauren;Cartmell, Alan;Wright, Gareth S. A.;Basle, Arnaud;Trost, Matthias;Henrissat, Bernard;Munoz-Munoz, Jose;Hirt, Robert P.;Kiessling, Laura L.;Lovering, Andrew L.;Williams, Spencer J.;Lowe, Elisabeth C.;Moynihan, Patrick J.
细菌细胞的生长和分裂需要合成和降解细胞壁聚合物的酶的协调作用。在这里，我们发现了一些酶，这些酶可以切割阿拉伯半乳聚糖的d-阿拉伯聚糖核心，这是结核分枝杆菌和其他分枝杆菌细胞壁的一种不寻常的成分。我们筛选了14种人类肠道来源的拟杆菌门，用于降解阿拉伯半乳聚糖，并鉴定了4个糖苷水解酶家族，这些酶对阿拉伯半乳聚糖的d-阿拉伯聚糖或d-半乳聚糖成分有活性。利用其中一个具有外d-半乳糖呋喃苷酶活性的分离株，我们产生了富集的d-阿拉伯糖，并利用它鉴定了一株Dysgonomonas gadei作为d-阿拉伯糖的降解者。这使得研究人员发现了可切割d-阿拉伯糖的内、外作用酶，包括DUF2961家族成员（GH172）和糖苷水解酶家族成员（DUF4185/GH183），这些酶具有内切d-阿拉伯糖的酶活性，并且在分枝杆菌和其他微生物中保守。分枝杆菌基因组编码两种保守的内切-阿拉伯糖酶，它们对含有d-阿拉伯糖的细胞壁成分阿拉伯半乳聚糖和脂阿拉伯糖甘露聚糖有不同的偏好，表明它们对细胞壁修饰和/或降解很重要。这些酶的发现将支持分枝杆菌细胞壁结构和功能的未来研究。细菌细胞的生长和分裂需要合成和降解细胞壁聚合物的酶的协调作用。在这里，作者发现了能够切割阿拉伯半乳聚糖d -阿拉伯聚糖核心的酶，这是结核分枝杆菌和其他分枝杆菌细胞壁的一种不寻常的成分。
Bacterial cell growth and division require the coordinated action of enzymes that synthesize and degrade cell wall polymers. Here, we identify enzymes that cleave the d-arabinan core of arabinogalactan, an unusual component of the cell wall of Mycobacterium tuberculosis and other mycobacteria. We screened 14 human gut-derived Bacteroidetes for arabinogalactan-degrading activities and identified four families of glycoside hydrolases with activity against the d-arabinan or d-galactan components of arabinogalactan. Using one of these isolates with exo-d-galactofuranosidase activity, we generated enriched d-arabinan and used it to identify a strain of Dysgonomonas gadei as a d-arabinan degrader. This enabled the discovery of endo- and exo-acting enzymes that cleave d-arabinan, including members of the DUF2961 family (GH172) and a family of glycoside hydrolases (DUF4185/GH183) that display endo-d-arabinofuranase activity and are conserved in mycobacteria and other microbes. Mycobacterial genomes encode two conserved endo-d-arabinanases with different preferences for the d-arabinan-containing cell wall components arabinogalactan and lipoarabinomannan, suggesting they are important for cell wall modification and/or degradation. The discovery of these enzymes will support future studies into the structure and function of the mycobacterial cell wall. Bacterial cell growth and division require the coordinated action of enzymes that synthesize and degrade cell wall polymers. Here, the authors identify enzymes that cleave the D-arabinan core of arabinogalactan, an unusual component of the cell wall of Mycobacterium tuberculosis and other mycobacteria.
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影响因子：
14.9
作者：
Letunic I;Bork P
通讯作者：
Bork P
DOI：
10.1016/j.jbc.2021.101324
发表时间：
2021-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kashima T;Okumura K;Ishiwata A;Kaieda M;Terada T;Arakawa T;Yamada C;Shimizu K;Tanaka K;Kitaoka M;Ito Y;Fujita K;Fushinobu S
通讯作者：
Fushinobu S
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
4.9
作者：
Brossier, Florence;Sougakoff, Wladimir;Veziris, Nicolas
通讯作者：
Veziris, Nicolas
饮食中的果糖聚糖会通过人类结肠菌属的协调酶途径降解。
影响因子：
28.3
作者：
Luis AS;Briggs J;Zhang X;Farnell B;Ndeh D;Labourel A;Baslé A;Cartmell A;Terrapon N;Stott K;Lowe EC;McLean R;Shearer K;Schückel J;Venditto I;Ralet MC;Henrissat B;Martens EC;Mosimann SC;Abbott DW;Gilbert HJ
通讯作者：
Gilbert HJ