Rapid enrichment of presynaptic protein in boutons undergoing classical conditioning is mediated by brain-derived neurotrophic factor.
Rapid enrichment of presynaptic protein in boutons undergoing classical conditioning is mediated by brain-derived neurotrophic factor.
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经典条件的胸子中突触前蛋白的快速富集是由脑衍生的神经营养因子介导的。
DOI：
10.1016/j.neuroscience.2011.12.015
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发表时间：
2012-02-17
期刊：
影响因子：
3.3
通讯作者：
Keifer, J.
中科院分区：
文献类型：
--
作者：
Li, W.;Keifer, J.
突触前结构改变被认为伴随着活动依赖的突触可塑性和学习。这可能涉及将非功能性突触转化为活性突触，或者产生全新的突触。在这里，我们使用经典条件反射的体外神经模拟，通过使用荧光示踪剂结合突触小泡相关蛋白突触素的免疫染色进行轨迹追踪，研究了仅限于传递条件刺激(CS)的听神经突触的突触前结构变化。结果表明，条件化处理后突触前听觉突触增大，突触素斑点染色面积增大，荧光强度增强。这只发生在与树突相对的听神经外突，而不是外展运动神经元的体细胞。条件反射增加了突触素免疫阳性突触素的百分比，并增加了它们所含突触素的数量。脑源性神经营养因子(BDNF)抗体可抑制条件反射诱导的这些结构变化。在条件化处理或脑源性神经营养因子治疗后，与外展运动神经元相对应的外展运动神经元的数量也有净增加。这些数据表明，在经典的条件反射过程中，突触前突触周围迅速富含神经递质循环和释放所需的蛋白质，这些过程是由BDNF介导的。
Presynaptic structural modifications are thought to accompany activity-dependent synaptic plasticity and learning. This may involve the conversion of nonfunctional synapses into active ones or the generation of entirely new synapses. Here, using an in vitro neural analog of classical conditioning, we investigated presynaptic structural changes restricted to auditory nerve synapses that convey the conditioned stimulus (CS) by tract tracing using fluorescent tracers combined with immunostaining for the synaptic vesicle-associated protein synaptophysin. The results show that the size of presynaptic auditory boutons increased and the area and fluorescence intensity of punctate staining for synaptophysin were enhanced after conditioning. This occurred only for auditory nerve boutons apposed to the dendrites but not the somata of abducens motor neurons. Conditioning increased the percentage of boutons that were immunopositive for synaptophysin and enhanced the number of synaptophysin puncta they contained. Pretreatment with antibodies against brain-derived neurotrophic factor (BDNF) inhibited these conditioning-induced structural changes. There was also a net increase in the number of boutons apposed to abducens motor neurons after conditioning or BDNF treatment. These data indicate that the rapid enrichment of presynaptic boutons with proteins required for neurotransmitter recycling and release occurs during classical conditioning and that these processes are mediated by BDNF.
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DOI：
10.1523/jneurosci.3649-09.2009
发表时间：
2009-11-25
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Keifer J;Sabirzhanov BE;Zheng Z;Li W;Clark TG
通讯作者：
Clark TG
影响因子：
16.2
作者：
Kwon SE;Chapman ER
通讯作者：
Chapman ER
影响因子：
3.7
作者：
Antonova, Irina;Lu, Fang-Min;Hawkins, Robert D.
通讯作者：
Hawkins, Robert D.
影响因子：
56.9
作者：
Kovalchuk, Y;Hanse, E;Konnerth, A
通讯作者：
Konnerth, A
影响因子：
56.9
作者：
Antonova, I;Arancio, O;Hawkins, RD
通讯作者：
Hawkins, RD