The complexity of human infected AIV H5N6 isolated from China.
The complexity of human infected AIV H5N6 isolated from China.
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从中国分离的人类感染的AIV H5N6的复杂性。
DOI：
10.1186/s12879-016-1932-1
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发表时间：
2016-10-25
影响因子：
3.7
通讯作者：
Jiang Q
中科院分区：
文献类型：
--
作者：
Zhang Z;Li R;Jiang L;Xiong C;Chen Y;Zhao G;Jiang Q
H7N9、H10N8和H5N6新型禽流感病毒目前在中国家禽群中流行，偶有感染人类和其他哺乳动物。2014-2015年中国人感染的禽流感病毒H5N6被认为是源自H6N6和H5的两个分支的三重重组病毒。目前的报告表明，其重组历史更为复杂。从NCBI流感病毒序列数据库和全球禽流感数据共享倡议中检索人感染禽流感病毒H5N6分离株的基因组。通过基本局部比对搜索工具获得了与基因组各片段具有高同源性的序列。使用mafft-7.037-win32程序进行校准；利用MEGA5.1/5.2/6.0软件构建了8棵大规模的、然后逐渐收敛的系统发育树。在中国传播的人感染AIV H5N6分离株的每个基因组片段演变成目前状态的事件可能发生在2013年之前，因此它们被重新组合成流行的AIV H5N6。A/Guangzhou/39715/2014（H5N6）和A/Sichuan/26221/2014（H5N6）的6个内部片段（PB2、PB1、PA、NP、NEP和M）是同源的，它们与HA同源于同一时期、同一地区的H5N1型AIV，而A/Yunnan/0127/2015（H5N6）的6个内部片段来源于2008年以来在华东地区流行的H9N2型AIV。2014-2015年从中国人、禽中分离的AIV H5N6具有异质性；H5N1和H9N2病毒都参与了中国H5N1病毒的重组。本文的在线版本（doi:10.1186/s12879-016-1932-1）包含补充材料，可供授权用户使用。
Novel avian influenza viruses (AIVs) of H7N9, H10N8, and H5N6 are currently circulating in China’s poultry flocks, occasionally infecting human and other mammals. Human infected AIV H5N6 in China during 2014–2015 is believed to be a triple reassortant originated from H6N6 and two clades of H5 viruses. The current report suggests that its reassortment history is more complicated. Genomes of human infected isolates of AIV H5N6 were searched from the NCBI Influenza Virus Sequence Database and the Global Initiative on Sharing Avian Influenza Data. Sequences shared high identities with each segment of their genomes were obtained through the Basic Local Alignment Search Tool. Alignments were done by mafft-7.037-win32 program; 8 large-scale and then 8 gradually converged phylogenetic trees were constructed by using MEGA5.1/5.2/6.0 Software. The events that each segment of the genomes of human infected AIV H5N6 isolates circulated in China had evolved into its current status might have happened before 2013, and so were they then reassorted into the epidemic AIV H5N6. A/Guangzhou/39715/2014(H5N6) and A/Sichuan/26221/2014(H5N6) had their six internal segments (PB2, PB1, PA, NP, NEP, and M) in common, and were reassorted from AIVs H5N1 in the same period and same region as that of HA, while A/Yunnan/0127/2015(H5N6) derived its six internal segments from AIV H9N2 that has been prevalent in Eastern China since 2008. AIV H5N6 isolates established from both human and poultry in China during 2014–2015 were heterogeneous; both AIVs H5N1 and H9N2 were involved in the reassortment of AIV H5N6 in China. The online version of this article (doi:10.1186/s12879-016-1932-1) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.85.2.324
发表时间：
1988-01-01
影响因子：
11.1
作者：
KAWAOKA, Y;WEBSTER, RG
通讯作者：
WEBSTER, RG
DOI：
10.1073/pnas.160270697
发表时间：
2000-08-15
影响因子：
11.1
作者：
Lin, YP;Shaw, M;Hay, A
通讯作者：
Hay, A
DOI：
10.1073/pnas.1422456112
发表时间：
2015-01-13
影响因子：
11.1
作者：
Pu, Juan;Wang, Shuoguo;Webster, Robert G.
通讯作者：
Webster, Robert G.
DOI：
10.1007/s11427-013-4496-y
发表时间：
2013-06
期刊：
Science China. Life sciences
影响因子：
--
作者：
Wu Y;Gao GF
通讯作者：
Gao GF
影响因子：
11.8
作者：
Wong FY;Phommachanh P;Kalpravidh W;Chanthavisouk C;Gilbert J;Bingham J;Davies KR;Cooke J;Eagles D;Phiphakhavong S;Shan S;Stevens V;Williams DT;Bounma P;Khambounheuang B;Morrissy C;Douangngeun B;Morzaria S
通讯作者：
Morzaria S