Identification of peptidomimetic telomere dysfunction inhibitor (TELODIN) through telomere dysfunction-induced foci (TIF) assay.
Identification of peptidomimetic telomere dysfunction inhibitor (TELODIN) through telomere dysfunction-induced foci (TIF) assay.
复制标题
通过端粒功能障碍诱导灶 (TIF) 测定鉴定拟肽端粒功能障碍抑制剂 (TELODIN)
DOI：
10.1016/j.xpro.2021.100620
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发表时间：
2021-09-17
期刊：
影响因子：
--
通讯作者：
Liu JP
中科院分区：
文献类型：
--
作者：
Wang L;Wang Z;Liu JP
Telomere dysfunction-induced focus (TIF) assay allows efficient profiling of telomere dysfunctions in cells and tissues. Here, we describe the use of the TIF assay to screen synthetic peptides from E3 ubiquitin ligase FBW7, a tumor suppressor gene product, to prevent TIFs caused by environmental radiation stress. We demonstrate peptidomimetic telomere dysfunction inhibitor as a potentially intervening therapeutic drug candidate in aging-related diseases. This work demonstrates a novel utility of the TIF assay protocol in identifying telomere dysfunction inhibitors. For complete details on the use and execution of this protocol, please refer to. Detailed experimental protocol for telomere dysfunction-induced focus (TIF) assay Optimized TIF assay for screening inhibitors of telomere dysfunction in cell cultures Identification of peptidomimetic telomere dysfunction inhibitor (TELODIN) from FBW7 Dose-dependent effect of TELODIN on TIFs caused by radiation stress in cancer cells Telomere dysfunction-induced focus (TIF) assay allows efficient profiling of telomere dysfunctions in cells and tissues. Here, we describe the use of the TIF assay to screen synthetic peptides from E3 ubiquitin ligase FBW7, a tumor suppressor gene product, to prevent TIFs caused by environmental radiation stress. We demonstrate peptidomimetic telomere dysfunction inhibitor as a potentially intervening therapeutic drug candidate in aging-related diseases. This work demonstrates a novel utility of the TIF assay protocol in identifying telomere dysfunction inhibitors.
Telomere dysfunction-induced focus (TIF) assay allows efficient profiling of telomere dysfunctions in cells and tissues. Here, we describe the use of the TIF assay to screen synthetic peptides from E3 ubiquitin ligase FBW7, a tumor suppressor gene product, to prevent TIFs caused by environmental radiation stress. We demonstrate peptidomimetic telomere dysfunction inhibitor as a potentially intervening therapeutic drug candidate in aging-related diseases. This work demonstrates a novel utility of the TIF assay protocol in identifying telomere dysfunction inhibitors. For complete details on the use and execution of this protocol, please refer to. Detailed experimental protocol for telomere dysfunction-induced focus (TIF) assay Optimized TIF assay for screening inhibitors of telomere dysfunction in cell cultures Identification of peptidomimetic telomere dysfunction inhibitor (TELODIN) from FBW7 Dose-dependent effect of TELODIN on TIFs caused by radiation stress in cancer cells Telomere dysfunction-induced focus (TIF) assay allows efficient profiling of telomere dysfunctions in cells and tissues. Here, we describe the use of the TIF assay to screen synthetic peptides from E3 ubiquitin ligase FBW7, a tumor suppressor gene product, to prevent TIFs caused by environmental radiation stress. We demonstrate peptidomimetic telomere dysfunction inhibitor as a potentially intervening therapeutic drug candidate in aging-related diseases. This work demonstrates a novel utility of the TIF assay protocol in identifying telomere dysfunction inhibitors.
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影响因子：
9.2
作者：
Takai, H;Smogorzewska, A;de Lange, T
通讯作者：
de Lange, T
DOI：
10.1007/978-1-4939-2963-4_14
发表时间：
2015-01-01
期刊：
IMMUNOSENESCENCE: METHODS AND PROTOCOLS
影响因子：
--
作者：
Rai, Rekha;Chang, Sandy
通讯作者：
Chang, Sandy
DOI：
10.1073/pnas.85.18.6622
发表时间：
1988-09-01
影响因子：
11.1
作者：
MOYZIS, RK;BUCKINGHAM, JM;WU, JR
通讯作者：
WU, JR
影响因子：
16.6
作者：
Nischan, Nicole;Herce, Henry D.;Hackenberger, Christian P. R.
通讯作者：
Hackenberger, Christian P. R.
DOI：
10.1007/978-1-4939-9432-8_11
发表时间：
2019-01-01
期刊：
RADIATION CYTOGENETICS
影响因子：
--
作者：
Cartwright, Ian M.;Haskins, Jeremy S.;Kato, Takamitsu A.
通讯作者：
Kato, Takamitsu A.