Artemisia afra and Artemisia annua Extracts Have Bactericidal Activity against Mycobacterium tuberculosis in Physiologically Relevant Carbon Sources and Hypoxia.
Artemisia afra and Artemisia annua Extracts Have Bactericidal Activity against Mycobacterium tuberculosis in Physiologically Relevant Carbon Sources and Hypoxia.
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DOI：
10.3390/pathogens12020227
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发表时间：
2023-02-01
期刊：
影响因子：
--
通讯作者：
Shell SS
中科院分区：
文献类型：
--
作者：
Kiani BH;Alonso MN;Weathers PJ;Shell SS
结核分枝杆菌（Mtb）是一种致命的病原体和人类结核病的病原体，每年导致约150万人死亡。这种病原体的耐药性不断增加，需要新的和改进的治疗策略。宿主-病原体相互作用的一个关键方面是细菌营养。在这项研究中，青蒿和青蒿二氯甲烷提取物进行了测试，在缺氧和各种感染相关的碳源（甘油，葡萄糖和胆固醇）对结核分枝杆菌菌株mc 26230的杀菌活性。无论碳源如何，两种提取物均显示出对Mtb的显著杀菌活性。根据致死曲线，A. afra对所有测试的碳源显示出最一致的抗Mtb杀菌活性，而A. annua在7 H9甘油基本培养基中表现出最高的杀菌活性。两种提取物在缺氧条件下均保留其对结核分枝杆菌的杀菌活性。需要进一步的研究来确定这些提取物的作用机制，并确定其活性成分化合物。
Mycobacterium tuberculosis (Mtb) is a deadly pathogen and causative agent of human tuberculosis, causing ~1.5 million deaths every year. The increasing drug resistance of this pathogen necessitates novel and improved treatment strategies. A crucial aspect of the host–pathogen interaction is bacterial nutrition. In this study, Artemisia annua and Artemisia afra dichloromethane extracts were tested for bactericidal activity against Mtb strain mc26230 under hypoxia and various infection-associated carbon sources (glycerol, glucose, and cholesterol). Both extracts showed significant bactericidal activity against Mtb, regardless of carbon source. Based on killing curves, A. afra showed the most consistent bactericidal activity against Mtb for all tested carbon sources, whereas A. annua showed the highest bactericidal activity in 7H9 minimal media with glycerol. Both extracts retained their bactericidal activity against Mtb under hypoxic conditions. Further investigations are required to determine the mechanism of action of these extracts and identify their active constituent compounds.
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DOI：
10.1038/s41579-018-0013-4
发表时间：
2018-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Ehrt S;Schnappinger D;Rhee KY
通讯作者：
Rhee KY
DOI：
10.1073/pnas.2201632119
发表时间：
2022-04-12
影响因子：
11.1
作者：
Koh, Eun-Ik;Oluoch, Peter O.;Ruecker, Nadine;Proulx, Megan K.;Soni, Vijay;Murphy, Kenan C.;Papavinasasundaram, Kadamba;Reames, Charlotte J.;Trujillo, Carolina;Zaveri, Anisha;Zimmerman, Matthew D.;Aslebagh, Roshanak;Baker, Richard E.;Shaffer, Scott A.;Guinn, Kristine M.;Fitzgerald, Michael;Dartois, Veronique;Ehrt, Sabine;Hung, Deborah T.;Ioerger, Thomas R.;Rubin, Eric J.;Rhee, Kyu Y.;Schnappinger, Dirk;Sassetti, Christopher M.
通讯作者：
Sassetti, Christopher M.
影响因子：
--
作者：
de Carvalho, Luiz Pedro S.;Fischer, Steven M.;Rhee, Kyu Y.
通讯作者：
Rhee, Kyu Y.
影响因子：
6.1
作者：
Di Pasqua, R;Hoskins, N;Mauriello, G
通讯作者：
Mauriello, G
影响因子：
3.6
作者：
Adler-Shohet, Felice C.;Low, Julie;Singh, Jasjit
通讯作者：
Singh, Jasjit