Ribosomal protein L4 of Lactobacillus rhamnosus LRB alters resistance to macrolides and other antibiotics.
Ribosomal protein L4 of Lactobacillus rhamnosus LRB alters resistance to macrolides and other antibiotics.
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DOI：
10.1111/omi.12281
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发表时间：
2020-06
影响因子：
3.7
通讯作者：
Biswas I
中科院分区：
文献类型：
--
作者：
Biswas S;Keightley A;Biswas I
鼠李糖乳杆菌是一种重要的乳酸菌，主要用作益生菌补充剂。这种细菌分泌益生菌特性所必需的免疫调节和抗菌肽。这种微生物还占据了不同的生态位，如胃肠道和口腔。一些研究表明L.鼠李糖酵母倾向于自发的基因组重排，而与生态起源无关。我们以前的特点是口服分离L。rhamnosus，LRB，其与广泛使用的益生菌菌株L.鼠李糖在这项研究中，我们分离出一种非靶向突变体，它对酸胁迫特别敏感。使用下一代测序，我们进一步绘制了基因组中的推定突变，并发现突变体在编码核糖体大亚基L4的rplD基因中获得了75个碱基对的缺失。该突变体在37 ° C和环境温度下具有生长缺陷。进一步的抗生素敏感性分析表明，该突变体对红霉素和氯霉素（两种靶向50S亚基的抗生素）的耐药性相对更强。相反，突变体对靶向30S亚基的四环素更敏感。因此，似乎非靶向突变可以显着改变抗生素耐药性的L。鼠李糖我们的研究引起了关注，益生菌使用L。应仔细监测鼠李糖，以避免意外后果。
Lactobacillus rhamnosus is an important lactic acid bacterium that is predominantly used as a probiotic supplement. This bacterium secretes immunomodulatory and antibacterial peptides that are necessary for the probiotic trait. This organism also occupies diverse ecological niches, such as gastrointestinal tracts and the oral cavity. Several studies have shown that L. rhamnosus is prone to spontaneous genome rearrangement irrespective of the ecological origins. We previously characterized an oral isolate of L. rhamnosus, LRB, which is genetically closely related to the widely used probiotic strain L. rhamnosus LGG. In this study, we isolated a nontargeted mutant that was particularly sensitive to acid stress. Using next generation sequencing, we further mapped the putative mutations in the genome and found that the mutant had acquired a deletion of 75 base pairs in the rplD gene that encodes the large ribosomal subunit L4. The mutant had a growth defect at 37°C and at ambient temperature. Further antibiotic sensitivity analyses indicated that the mutant is relatively more resistant to erythromycin and chloramphenicol; two antibiotics that target the 50S subunit. In contrast, the mutant was more sensitive to tetracycline, which targets the 30S subunit. Thus, it appears that nontargeted mutations could significantly alter the antibiotic resistance profile of L. rhamnosus. Our study raises concern that probiotic use of L. rhamnosus should be carefully monitored to avoid unintended consequences.
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影响因子：
5.6
作者：
Banna GL;Torino F;Marletta F;Santagati M;Salemi R;Cannarozzo E;Falzone L;Ferraù F;Libra M
通讯作者：
Libra M
影响因子：
3.7
作者：
Biswas, Saswati;Keightley, Andrew;Biswas, Indranil
通讯作者：
Biswas, Indranil
影响因子：
2.6
作者：
Mayrhofer, Sigrid;Zitz, Ulrike;Domig, Konrad J.
通讯作者：
Domig, Konrad J.
影响因子：
5.2
作者：
Kwon, Ye Won;Bae, Jae-Han;Han, Nam Soo
通讯作者：
Han, Nam Soo
影响因子：
4.5
作者：
Halpern, David;Chiapello, Helene;El Karoui, Meriem
通讯作者：
El Karoui, Meriem